3b-Hydroxy-5-cholenoic acid 

3β-Hydroxy-5-cholenoic acid is a steroidal monohydroxy bile acid and serves as a substrate for sulfation reactions. It is applicable to the research of extrahepatic biliary atresia and recurrent intrahepatic cholestasis of pregnancy^[1][2].

3b-Hydroxy-5-cholenoic acid (compound 15) (up to 10 μM; 24 h) does not act as a pharmacological chaperone for NPC1L1^{L1072T/L1168I} in HEK293 cells, with no observed correction of the mutant protein's ER localization defect at concentrations up to 10 μM for 24 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

374.56

C₂₄H₃₈O₃

5255-17-4

Solid

White to off-white

C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Karaki F, et al. Structure-activity relationship studies of Niemann-Pick type C1-like 1 (NPC1L1) ligands identified by screening assay monitoring pharmacological chaperone effect. Bioorg Med Chem. 2013;21(17):5297-5309.  [Content Brief]

  [2]. Back P, et al. Identification of 3 beta-hydroxy-5-cholenoic acid in human meconium. Hoppe Seylers Z Physiol Chem. 1973 Jan;354(1):83-9  [Content Brief]