3b-Hydroxy-5-cholenoic acid
Based on 1 publication(s) in Google Scholar
3β-Hydroxy-5-cholenoic acid is a steroidal monohydroxy bile acid and serves as a substrate for sulfation reactions. It is applicable to the research of extrahepatic biliary atresia and recurrent intrahepatic cholestasis of pregnancy.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 5255-17-4
- Formula: C24H38O3
- Molecular Weight:374.56
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) 3b-Hydroxy-5-cholenoic acid
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
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| HEK293 | EC50 |
>10 μM
Compound: 15
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Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
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[PMID: 23830695] |
3b-Hydroxy-5-cholenoic acid (compound 15) (up to 10 μM; 24 h) does not act as a pharmacological chaperone for NPC1L1L1072T/L1168I in HEK293 cells, with no observed correction of the mutant protein's ER localization defect at concentrations up to 10 μM for 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5255-17-4
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Appearance Solid
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Molecular Weight 374.56
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Formula C24H38O3
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Color White to off-white
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SMILES
C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 5 mg/mL (13.35 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 4 mg/mL (10.68 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Karaki F, et al. Structure-activity relationship studies of Niemann-Pick type C1-like 1 (NPC1L1) ligands identified by screening assay monitoring pharmacological chaperone effect. Bioorg Med Chem. 2013;21(17):5297-5309. [Content Brief]
[2]. Back P, et al. Identification of 3 beta-hydroxy-5-cholenoic acid in human meconium. Hoppe Seylers Z Physiol Chem. 1973 Jan;354(1):83-9 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 2.6698 mL | 13.3490 mL | 26.6980 mL | 66.7450 mL |
| 5 mM | 0.5340 mL | 2.6698 mL | 5.3396 mL | 13.3490 mL | |
| 10 mM | 0.2670 mL | 1.3349 mL | 2.6698 mL | 6.6745 mL |
3b-Hydroxy-5-cholenoic acid Related Classifications
HY-113315 Related Classifications
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- 3b-Hydroxy-5-cholenoic acid
- 5255-17-4
- Endogenous Metabolite
- mature neonates
- Niemann-Pick type C1-like 1
- cholesterol catabolism pathway
- NPC1L1 L1072T/L1168I mutant
- steroidal monohydroxy bile acid
- extrahepatic biliary atresia
- premature neonates
- sulfate esterification
- human meconium
- recurrent cholestasis of pregnancy
- Inhibitor
- inhibitor
- inhibit