4-Methylcatechol

Name: 4-Methylcatechol
Brand: MedChemExpress
SKU: HY-W012814
Rating: 4.5 (0 reviews)

Cat. No.: HY-W012814 Purity: 99.17%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

4-Methylcatechol is an intermediate in the degradation of some alkylbenzenes and an orally active suicide inhibitor of catechol 2,3-dioxygenase (C23O). 4-Methylcatechol induces apoptosis in melanoma cells through oxidative stress, but some studies have also shown that 4-Methylcatechol is carcinogenic. In addition, 4-Methylcatechol has antiplatelet and blood pressure-lowering activities. 4-Methylcatechol can also inhibit protein oxidation in beef but does not disulfide formation.

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4-Methylcatechol 화학구조

CAS No. : 452-86-8

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
100 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	해외재고보유	Estimated Time of Arrival: December 31
10 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Other Forms of 4-Methylcatechol:

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

4-Methylcatechol (0-25 μg/mL; 8 h-14 days) can inhibit the proliferation of melanoma cells, induce cell cycle arrest and induce apoptosis through the generation of reactive oxygen species through mitochondrial pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

4-Methylcatechol (Feed management; 2%; 104 weeks) is carcinogenic to the forestomach and glandular stomach in F344 rats^[2].
4-Methylcatechol (Intravenous injection; 0.2-25 mg/kg; single dose) has vasodilatory and blood pressure-lowering effects in spontaneously hypertensive rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male and female F344 rats aged 5 weeks old^[2]
Dosage:	2%
Administration:	Feed management; 104 weeks
Result:	Slightly reduced the average body weight and food intake in rats. Decreased survival from about the 70th week in male rats, the cause of death may have been perforation due to glandular stomach ulceration. Caused multiple nodules or large masses in the forestomachs in rats. Caused thickening or polypoid lesions in the pyloric region in the glandular stomach in rats. Induced forestomach papillomas and squamous-cell carcinomas, as well as glandular stomach submucosal hyperplasias, adenomas, and adenocarcinomas, some of which were accompanied by ulceration or erosion.

분자량

124.14

화학식

C₇H₈O₂

CAS No.

452-86-8

Appearance

Solid

Color

Off-white to light brown

SMILES

OC1=CC=C(C)C=C1O

Structure Classification

Initial Source

Microorganisms

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (805.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (805.55 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	8.0555 mL	40.2774 mL	80.5549 mL
5 mM	1.6111 mL	8.0555 mL	16.1110 mL
10 mM	0.8055 mL	4.0277 mL	8.0555 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (20.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 99.52%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (249 KB) HNMR (111 KB) RP-HPLC (192 KB)

Handling Instructions (2659 KB)

References

[1]. Payton F, et al. 4-Methylcatechol-induced oxidative stress induces intrinsic apoptotic pathway in metastatic melanoma cells. Biochem Pharmacol. 2011 May 15;81(10):1211-8. [Content Brief]

[2]. Asakawa E, et al. Carcinogenicity of 4-methoxyphenol and 4-methylcatechol in F344 rats. Int J Cancer. 1994 Jan 2;56(1):146-52. [Content Brief]

[3]. Pourová J, et al. Two flavonoid metabolites, 3,4-dihydroxyphenylacetic acid and 4-methylcatechol, relax arteries ex vivo and decrease blood pressure in vivo. Vascul Pharmacol. 2018 Dec;111:36-43. [Content Brief]

[4]. Okuta A, et al. Construction of chimeric catechol 2,3-dioxygenase exhibiting improved activity against the suicide inhibitor 4-methylcatechol. Appl Environ Microbiol. 2004 Mar;70(3):1804-10. [Content Brief]

[5]. Jongberg S, et al. 4-methylcatechol inhibits protein oxidation in meat but not disulfide formation. J Agric Food Chem. 2011 Sep 28;59(18):10329-35. [Content Brief]

[6]. Applová L, et al. 4-Methylcatechol, a Flavonoid Metabolite with Potent Antiplatelet Effects. Mol Nutr Food Res. 2019 Aug 7;63(20):e1900261. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	8.0555 mL	40.2774 mL	80.5549 mL	201.3872 mL
	5 mM	1.6111 mL	8.0555 mL	16.1110 mL	40.2774 mL
	10 mM	0.8055 mL	4.0277 mL	8.0555 mL	20.1387 mL
	15 mM	0.5370 mL	2.6852 mL	5.3703 mL	13.4258 mL
	20 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0694 mL
	25 mM	0.3222 mL	1.6111 mL	3.2222 mL	8.0555 mL
	30 mM	0.2685 mL	1.3426 mL	2.6852 mL	6.7129 mL
	40 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0347 mL
	50 mM	0.1611 mL	0.8055 mL	1.6111 mL	4.0277 mL
	60 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3565 mL
	80 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	100 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.