3,4-Dihydroxyphenylglycol
Based on 1 Customer Validation
3,4-Dihydroxyphenylglycol is a phenolic compound and antioxidant. 3,4-Dihydroxyphenylglycol can be extracted from olive oil. 3,4-Dihydroxyphenylglycol downregulates the expression of proinflammatory enzymes (iNOS, COX-2) and prevents the degradation of IκBα. 3,4-Dihydroxyphenylglycol has antioxidant properties. 3,4-Dihydroxyphenylglycol has shown positive effects on frozen-thawed ram sperm. 3,4-Dihydroxyphenylglycol can be used in the study of inflammatory-related diseases (such as ulcerative colitis) and reproductive health (sperm cryopreservation).
For research use only. We do not sell to patients.
- Purity: 98.07%
- CAS No.: 28822-73-3
- Formula: C8H10O4
- Molecular Weight:170.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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COX-2 |
3,4-Dihydroxyphenylglycol (50-100 μM) reduces nitrite production, downregulates iNOS and COX-2 expression, and prevents IκBα degradation in LPS-stimulated mouse peritoneal macrophages[1].
3,4-Dihydroxyphenylglycol exhibits antioxidant activity with an IC50 of 1.55 μM in a DPPH free radical scavenging assay[1].
3,4-Dihydroxyphenylglycol (10-70 μg/mL) exhibits positive effects on frozen-thawed ram spermatozoa, reducing LPO[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 28822-73-3
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Appearance Solid
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Molecular Weight 170.16
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Formula C8H10O4
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Color Off-white to light yellow
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SMILES
C1=CC(=C(C=C1C(CO)O)O)O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (587.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Aparicio-Soto M, et al. Naturally occurring hydroxytyrosol derivatives: hydroxytyrosyl acetate and 3,4-dihydroxyphenylglycol modulate inflammatory response in murine peritoneal macrophages. Potential utility as new dietary supplements. J Agric Food Chem. 2015 Jan 28;63(3):836-46. [Content Brief]
[2]. Arando A, et al. Effect of different olive oil-derived antioxidants (hydroxytyrosol and 3,4-dihydroxyphenylglycol) on the quality of frozen-thawed ram sperm. Cryobiology. 2019 Feb;86:33-39. [Content Brief]
[3]. Sánchez-Fidalgo S, et al. Effects of dietary virgin olive oil polyphenols: hydroxytyrosyl acetate and 3, 4-dihydroxyphenylglycol on DSS-induced acute colitis in mice. J Nutr Biochem. 2015 May;26(5):513-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.8768 mL | 29.3841 mL | 58.7682 mL | 146.9205 mL |
| 5 mM | 1.1754 mL | 5.8768 mL | 11.7536 mL | 29.3841 mL | |
| 10 mM | 0.5877 mL | 2.9384 mL | 5.8768 mL | 14.6921 mL | |
| 15 mM | 0.3918 mL | 1.9589 mL | 3.9179 mL | 9.7947 mL | |
| 20 mM | 0.2938 mL | 1.4692 mL | 2.9384 mL | 7.3460 mL | |
| 25 mM | 0.2351 mL | 1.1754 mL | 2.3507 mL | 5.8768 mL | |
| 30 mM | 0.1959 mL | 0.9795 mL | 1.9589 mL | 4.8974 mL | |
| 40 mM | 0.1469 mL | 0.7346 mL | 1.4692 mL | 3.6730 mL | |
| 50 mM | 0.1175 mL | 0.5877 mL | 1.1754 mL | 2.9384 mL | |
| 60 mM | 0.0979 mL | 0.4897 mL | 0.9795 mL | 2.4487 mL | |
| 80 mM | 0.0735 mL | 0.3673 mL | 0.7346 mL | 1.8365 mL | |
| 100 mM | 0.0588 mL | 0.2938 mL | 0.5877 mL | 1.4692 mL |
3,4-Dihydroxyphenylglycol Related Classifications
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