1. Apoptosis
    Metabolic Enzyme/Protease
  2. Apoptosis
    Drug Metabolite
  3. 5β-Dihydrocortisol

5β-Dihydrocortisol 

Cat. No.: HY-N3995
Handling Instructions

5β-Dihydrocortisol, a metabolite of Cortisol, is a potential mineralocorticoid. 5β-Dihydrocortisol can potentiate glucocorticoid activity in raising the intraocular pressure. 5β-Dihydrocortisol causes breast cancer cell apoptosis.

For research use only. We do not sell to patients.

5β-Dihydrocortisol Chemical Structure

5β-Dihydrocortisol Chemical Structure

CAS No. : 1482-50-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 308 In-stock
Estimated Time of Arrival: December 31
5 mg USD 280 In-stock
Estimated Time of Arrival: December 31
10 mg USD 470 In-stock
Estimated Time of Arrival: December 31
25 mg USD 850 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

5β-Dihydrocortisol, a metabolite of Cortisol, is a potential mineralocorticoid. 5β-Dihydrocortisol can potentiate glucocorticoid activity in raising the intraocular pressure. 5β-Dihydrocortisol causes breast cancer cell apoptosis[1][2][3][4][5].

In Vitro

5β-Dihydrocortisol (10-100 μM; 48 h) inhibits the viability of MCF-7 cells, with an IC50 of 27.59 μM[2].
5β-Dihydrocortisol (14 μM; 24 h) induces 35.6% early apoptosis and 2.5% late apoptosis in MCF-7 cells[2].
5β-Dihydrocortisol (1-10 μM; 48 h) quenches the intrinsic fluorescence of human serum albumin (HAS) with a maximum emission peak at 360 nm, with no shift in fluorescence peak[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: MCF-7 and HEK 293 cells
Concentration: 10, 20, 40, 60, 80, 100 μM
Incubation Time: 48 hours
Result: Inhibited the viability of MCF-7 cells in a dose-dependent manner.
No toxicity in terms of cell viability was observed with HEK293 cell line.

Apoptosis Analysis[2]

Cell Line: MCF-7 cells
Concentration: 14 μM
Incubation Time: 24 hours
Result: Induced 35.6% and 2.5% of early and late apoptosis.
In Vivo

5β-Dihydrocortisol (0.1-1.0%; 18 d) potentiates the action of topically applied Dexamethasone (0.06%) in raising the intraocular pressure (IOP) in young rabbits[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

364.48

Formula

C₂₁H₃₂O₅

CAS No.

1482-50-4

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (342.95 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7436 mL 13.7182 mL 27.4363 mL
5 mM 0.5487 mL 2.7436 mL 5.4873 mL
10 mM 0.2744 mL 1.3718 mL 2.7436 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.71 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.71 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.71 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

5β-DihydrocortisolApoptosisDrug MetaboliteCortisolmineralocorticoidglucocorticoidintraocularpressurebreastcancerapoptosisInhibitorinhibitorinhibit

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5β-Dihydrocortisol
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