5'-Amino-5'-deoxyadenosine
Based on 1 Customer Validation
5'-Amino-5'-deoxyadenosine (NH2dAdo; Nsc 238990) is a purine nucleoside analog and a 5'-deoxyadenosine derivative. 5'-Amino-5'-deoxyadenosine inhibits vaccinia virus (CV) proliferation in cultured cells. 5'-Amino-5'-deoxyadenosine can be used in research on vaccinia virus infection.
For research use only. We do not sell to patients.
- Purity : 96.40%
- CAS No.: 14365-44-7
- Formula: C10H14N6O3
- Molecular Weight:266.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-B1449
UridineNucleoside -
HY-103185
CCPAA1 Adenosine Receptor Agonist -
HY-B1449R
Uridine (Standard)Nucleoside -
HY-W017163
7-MethylxanthineAdenosine Receptor Antagonist -
HY-W009538
5'-Deoxy-5-fluorocytidineMetabolite Of Capecitabine -
HY-116887
N6-MethyladeninePurine -
HY-123523
EnocitabineDNA Chain Terminator -
HY-W009538R
5'-Deoxy-5-fluorocytidine (Standard)Standard Of 5'-Deoxy-5-fluorocytidine -
HY-116887R
N6-Methyladenine (Standard)Purine -
HY-W012293
3'-DeoxycytidinePurine Nucleoside Analog -
HY-103185R
CCPA (Standard)Standard of CCPA -
HY-139099
Diguanosine 5′-triphosphateDinucleoside Triphosphates
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| IMR-32 | IC50 |
6330 nM
Compound: 1
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Inhibition of adenosine phosphorylation in confluent IMR-32 (human neuroblastoma) cells.
Inhibition of adenosine phosphorylation in confluent IMR-32 (human neuroblastoma) cells.
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[PMID: 11405650] |
In Vitro
5'-Amino-5'-deoxyadenosine (Compound 12) (12-200 μg/mL; 48 h) partially inhibits vaccinia virus plaque formation in Vero cell cultures in vitro in a concentration-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 14365-44-7
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Appearance Solid
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Molecular Weight 266.26
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Formula C10H14N6O3
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Color Off-white to light yellow
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SMILES
O[C@@H]1[C@@H](CN)O[C@@H](N2C(N=CN=C3N)=C3N=C2)[C@@H]1O
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Synonyms
NH2dAdo; Nsc 238990
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 200 mg/mL (751.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (290 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Grytzmann B, et al. Derivatives of 3'- and 5'-deoxyadenosine: their inhibitory activity against DNA viruses. Chemotherapy. 1980;26(5):316-22. [Content Brief]
[2]. Robak T, et al. Purine nucleoside analogs in the treatment of rarer chronic lymphoid leukemias. Current pharmaceutical design. 2012;18(23):3373-88. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.7557 mL | 18.7786 mL | 37.5573 mL | 93.8932 mL |
| 5 mM | 0.7511 mL | 3.7557 mL | 7.5115 mL | 18.7786 mL | |
| 10 mM | 0.3756 mL | 1.8779 mL | 3.7557 mL | 9.3893 mL | |
| 15 mM | 0.2504 mL | 1.2519 mL | 2.5038 mL | 6.2595 mL | |
| 20 mM | 0.1878 mL | 0.9389 mL | 1.8779 mL | 4.6947 mL | |
| 25 mM | 0.1502 mL | 0.7511 mL | 1.5023 mL | 3.7557 mL | |
| 30 mM | 0.1252 mL | 0.6260 mL | 1.2519 mL | 3.1298 mL | |
| 40 mM | 0.0939 mL | 0.4695 mL | 0.9389 mL | 2.3473 mL | |
| 50 mM | 0.0751 mL | 0.3756 mL | 0.7511 mL | 1.8779 mL | |
| 60 mM | 0.0626 mL | 0.3130 mL | 0.6260 mL | 1.5649 mL | |
| 80 mM | 0.0469 mL | 0.2347 mL | 0.4695 mL | 1.1737 mL | |
| 100 mM | 0.0376 mL | 0.1878 mL | 0.3756 mL | 0.9389 mL |
5'-Amino-5'-deoxyadenosine Related Classifications
HY-131818 Related Classifications
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Keywords
- 5'-Amino-5'-deoxyadenosine
- 14365-44-7
- NH2dAdo
- Nsc 238990
- Nsc238990
- Nsc 238990
- Nsc-238990
- Nucleoside Antimetabolite/Analog
- Orthopoxvirus
- agar diffusion assay
- HeLa cell cultures
- vaccinia virus plaque formation
- adenosine deaminase
- Vero cell cultures
- 5'-deoxyadenosine derivative
- purine nucleoside analog
- vaccinia virus
- herpesvirus hominis type 1
- adenovirus 5
- Inhibitor
- inhibitor
- inhibit