5'-Deoxy-5-fluorocytidine
Based on 1 Customer Validation
5'-Deoxy-5-fluorocytidine (5-Fluoro-5'-deoxycytidine) is a cytidine analog and metabolite of Capecitabine (HY-B0016). 5'-Deoxy-5-fluorocytidine is converted from Capecitabine by carboxylesterase in the liver. 5'-Deoxy-5-fluorocytidine is deaminated by cytidine deaminase to generate 5'-deoxy-5-fluorouridine, which is finally converted into 5-fluorouracil (HY-90006) by thymidine phosphorylase in tumor tissues to exert anti-tumor effects. 5'-Deoxy-5-fluorocytidine is used in the researches for solid tumors such as colorectal cancer, non-small cell lung cancer and breast cancer.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.91%
- CAS. Nr.: 66335-38-4
- Formel: C9H12FN3O4
- Molecular Weight:245.21
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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HY-B0016
CapecitabineDNA/RNA Synthesis Inhibitor -
HY-90006
5-FluorouracilThymidylate Synthase Inhibitor -
HY-B0021
DoxifluridineNucleoside Antimetabolite/Analog Inhibitor -
HY-15345A
TetrahydrouridineCDA Inhibitor -
HY-W009538R
5'-Deoxy-5-fluorocytidine (Standard)Standard Of 5'-Deoxy-5-fluorocytidine -
HY-B0003
Gemcitabine hydrochlorideDNA Synthesis Inhibitor -
HY-10586
5-AzacytidineDNMT Inhibitor -
HY-B0016R
Capecitabine (Standard)DNA/RNA Synthesis Inhibitor -
HY-A0061
TrifluridineAnti-Cancer Agent/Anti-Viral Agent -
HY-118411
5-Ethynyl-2'-deoxyuridinePROTAC Linkers -
HY-10586R
5-Azacytidine (Standard)DNMT Inhibitor -
HY-N0091
HypoxanthineEndogenous Metabolite
Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Beschreibung
In Vitro
5'-Deoxy-5-fluorocytidine (200 μM) significantly inhibits the proliferation of chemotherapy-resistant A549 paraclone cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 66335-38-4
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Appearance Solid
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Molecular Weight 245.21
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Formel C9H12FN3O4
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Color White to off-white
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SMILES
O=C(N=C(N)C(F)=C1)N1[C@@H]2O[C@H](C)[C@@H](O)[C@H]2O
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Synonyms
5-Fluoro-5'-deoxycytidine
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (407.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Gowher H, et al. Mechanism of inhibition of DNA methyltransferases by cytidine analogs in cancer therapy. Cancer Biol Ther. 2004 Nov;3(11):1062-8. [Content Brief]
[2]. Peters GJ, et al. Lipophilic prodrugs and formulations of conventional (deoxy)nucleoside and fluoropyrimidine analogs in cancer. Nucleosides Nucleotides Nucleic Acids. 2011 Dec;30(12):1168-80. [Content Brief]
[3]. Miwa M, et al. Design of a novel oral fluoropyrimidine carbamate, capecitabine, which generates 5-fluorouracil selectively in tumours by enzymes concentrated in human liver and cancer tissue. Eur J Cancer. 1998 Jul;34(8):1274-81. [Content Brief]
[4]. Gao Y, et al. Chemotherapy-induced CDA expression renders resistant non-small cell lung cancer cells sensitive to 5'-deoxy-5-fluorocytidine (5'-DFCR). J Exp Clin Cancer Res. 2021 Apr 19;40(1):138. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.0781 mL | 20.3907 mL | 40.7814 mL | 101.9534 mL |
| 5 mM | 0.8156 mL | 4.0781 mL | 8.1563 mL | 20.3907 mL | |
| 10 mM | 0.4078 mL | 2.0391 mL | 4.0781 mL | 10.1953 mL | |
| 15 mM | 0.2719 mL | 1.3594 mL | 2.7188 mL | 6.7969 mL | |
| 20 mM | 0.2039 mL | 1.0195 mL | 2.0391 mL | 5.0977 mL | |
| 25 mM | 0.1631 mL | 0.8156 mL | 1.6313 mL | 4.0781 mL | |
| 30 mM | 0.1359 mL | 0.6797 mL | 1.3594 mL | 3.3984 mL | |
| 40 mM | 0.1020 mL | 0.5098 mL | 1.0195 mL | 2.5488 mL | |
| 50 mM | 0.0816 mL | 0.4078 mL | 0.8156 mL | 2.0391 mL | |
| 60 mM | 0.0680 mL | 0.3398 mL | 0.6797 mL | 1.6992 mL | |
| 80 mM | 0.0510 mL | 0.2549 mL | 0.5098 mL | 1.2744 mL | |
| 100 mM | 0.0408 mL | 0.2039 mL | 0.4078 mL | 1.0195 mL |
5'-Deoxy-5-fluorocytidine Related Classifications
HY-W009538 Related Classifications
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