5-Hydroxypyrazine-2-Carboxylic Acid
Based on 1 publication(s) in Google Scholar
5-Hydroxypyrazine-2-Carboxylic Acid is a dibasic acid and also a urinary metabolite of coffee-derived 2-methylpyrazine (HY-W067358). 5-Hydroxypyrazine-2-Carboxylic Acid serves as an intermediate in the production of anti-tuberculosis reagents. It can be used in research related to tuberculosis[1][2].
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 34604-60-9
- Formula: C5H4N2O3
- Molecular Weight:140.10
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-N0264
Ligustrazine
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HY-B0271
Pyrazinamide
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HY-34439
2,5-Dimethylpyrazine
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HY-B0271R
Pyrazinamide (Standard)
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HY-W067358
2-Methylpyrazine
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HY-W017141R
2-Isobutyl-3-methoxypyrazine (Standard)
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HY-59261
2-Amino-5-bromopyrazine
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HY-W017140
2-Sec-butyl-3-methoxypyrazine
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HY-W040790
2,6-Dimethylpyrazine
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HY-W040948
2-Ethylpyrazine
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HY-Y0131
Pyrazine-2-carbonitrile
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HY-W017141
2-Isobutyl-3-methoxypyrazine
Publications Citing Use of MedChemExpress (MCE) 5-Hydroxypyrazine-2-Carboxylic Acid
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Biological Activity
5-Hydroxypyrazine-2-Carboxylic Acid (2-2.5 mmol; 280-350 mg in 50 mL water) (purity 99.54%) is a diprotic acid with pKa1 = 3.42 and pKa2 = 7.96, and exhibits enhanced buffer capacity at pH ~3.5 and ~8[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 34604-60-9
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Appearance Solid
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Molecular Weight 140.10
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Formula C5H4N2O3
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SMILES
O=C(O)C1=NC=C(O)N=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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PLoS One
Immunological detection of pyrazine-2-carboxylic acid for the detection of pyrazinamide resistance in Mycobacterium tuberculosis. [Abstract]2020 Nov 5;15(11):e0241600. PMID: 33151985
Solvent & Solubility
DMSO : 50 mg/mL (356.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 4 mg/mL (28.55 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sak-Bosnar M, et al. Acid-base characterization of 5-hydroxypyrazine-2-carboxylic acid and the role of ionic equilibria in the optimization of some process conditions for its biocatalytic production. Anal Bioanal Chem. 2005 Oct;383(3):539-45. [Content Brief]
[2]. Kremer JI, et al. Alkylpyrazines from Coffee are Extensively Metabolized to Pyrazine Carboxylic Acids in the Human Body. Mol Nutr Food Res. 2019 Jul;63(14):e1801341. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 7.1378 mL | 35.6888 mL | 71.3776 mL | 178.4440 mL |
| 5 mM | 1.4276 mL | 7.1378 mL | 14.2755 mL | 35.6888 mL | |
| 10 mM | 0.7138 mL | 3.5689 mL | 7.1378 mL | 17.8444 mL | |
| 15 mM | 0.4759 mL | 2.3793 mL | 4.7585 mL | 11.8963 mL | |
| 20 mM | 0.3569 mL | 1.7844 mL | 3.5689 mL | 8.9222 mL | |
| 25 mM | 0.2855 mL | 1.4276 mL | 2.8551 mL | 7.1378 mL | |
| DMSO | 30 mM | 0.2379 mL | 1.1896 mL | 2.3793 mL | 5.9481 mL |
| 40 mM | 0.1784 mL | 0.8922 mL | 1.7844 mL | 4.4611 mL | |
| 50 mM | 0.1428 mL | 0.7138 mL | 1.4276 mL | 3.5689 mL | |
| 60 mM | 0.1190 mL | 0.5948 mL | 1.1896 mL | 2.9741 mL | |
| 80 mM | 0.0892 mL | 0.4461 mL | 0.8922 mL | 2.2305 mL | |
| 100 mM | 0.0714 mL | 0.3569 mL | 0.7138 mL | 1.7844 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
5-Hydroxypyrazine-2-Carboxylic Acid Related Classifications
HY-76210 Related Classifications
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