5-JOE BCN
5-JOE BCN is a derivative of JOE featuring a BCN (bicyclo[6.1.0]nonyne) group, designed for copper-free click chemistry reactions. 5-JOE BCN can be used for copper-free click chemistry-based bioconjugation (Ex/Em = 520/548 nm) .
For research use only. We do not sell to patients.
- Formula: C44H48Cl2N2O13
- Molecular Weight:883.76
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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Molecular Weight 883.76
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Formula C44H48Cl2N2O13
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SMILES
ClC1=C(O)C(OC)=CC2=C1OC(C(Cl)=C3O)=C(C=C3OC)C24C5=CC=C(C(NCCCOCCOCCOCCCNC(OC[C@H]6[C@@]7(CCC#CCC[C@]67[H])[H])=O)=O)C=C5C(O4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
5-JOE BCN Related Classifications
HY-D3505 Related Classifications
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