1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis
  3. 6-Thio-2'-Deoxyguanosine

6-Thio-2'-Deoxyguanosine (Synonyms: 6-thio-dG; β-TGdR)

Cat. No.: HY-18762 Purity: >97.0%
Handling Instructions

6-Thio-2'-Deoxyguanosine is a nucleoside analogue that can be incorporated into de novo-synthesized telomeres by telomerase.

For research use only. We do not sell to patients.

6-Thio-2'-Deoxyguanosine Chemical Structure

6-Thio-2'-Deoxyguanosine Chemical Structure

CAS No. : 789-61-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 77 In-stock
Estimated Time of Arrival: December 31
5 mg USD 70 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
25 mg USD 220 In-stock
Estimated Time of Arrival: December 31
50 mg USD 390 In-stock
Estimated Time of Arrival: December 31
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Description

6-Thio-2'-Deoxyguanosine is a nucleoside analogue that can be incorporated into de novo-synthesized telomeres by telomerase.

IC50 & Target

DNA/RNA Synthesis[1]

In Vitro

Treatment with 6-Thio-2'-Deoxyguanosine results in rapid cell death for the vast majority of the cancer cell lines tested with IC50s of between 0.7-2.9 µM[1].

In Vivo

In A549 lung cancer cell-based mouse xenograft studies, 6-Thio-2'-Deoxyguanosine causes a decrease of the tumor growth rate, superior to that observed with 6-thioguanine treatment. Additionally, 6-Thio-2'-Deoxyguanosine increases telomere dysfunction in tumor cells in vivo[1].

Molecular Weight

283.31

Formula

C₁₀H₁₃N₅O₃S

CAS No.

789-61-7

SMILES

OC[[email protected]@H]1[[email protected]](C[[email protected]](N2C=NC3=C2NC(N)=NC3=S)O1)O

Shipping

Room temperature in continental US; may vary elsewhere

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 130 mg/mL (458.86 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5297 mL 17.6485 mL 35.2970 mL
5 mM 0.7059 mL 3.5297 mL 7.0594 mL
10 mM 0.3530 mL 1.7649 mL 3.5297 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

HCT116, A549, and H2882, HCC2429, HCC827, HCC15, H2087, HCC4017, HCC515, H2009, BJ and HCEC1 cells are plated in growth media in 96 well plates. Cells are incubated for 1 week and treated with varying concentrations of 6-Thio-2'-Deoxyguanosine and 6-thioguanine or DMSO every three days. The 96 well plates are analyzed for the CellTiterGlo luminescent cell viability assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Athymic NCR nu/nu female mice (6 weeks old) are used. A549 cells are inoculated subcutaneously into the left and right dorsal flanks of the nude mice in 100 µL phosphate buffered saline (PBS). When tumors reach 40 mm3 average volume, mice are randomly divided into control, 6-thio-dG and 6-thioguanine treatment groups (3 animals in each group). Animals are injected intraperitoneally every two days for 17 days at a dose of 2mg/kg in 100 µL DMSO/PBS mixture per mouse. In addition, different animals are injected intratumorally every day for 16 days at a dose of Athymic NCR nu/nu female mice2.5mg/kg in 50 µL DMSO/PBS mixture per mouse. Tumor size is measured by calipers and recorded either every day or every two days[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >97.0%

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Product Name:
6-Thio-2'-Deoxyguanosine
Cat. No.:
HY-18762
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