1. Immunology/Inflammation Vitamin D Related/Nuclear Receptor
  2. Glucocorticoid Receptor
  3. Methylprednisolone succinate sodium

Methylprednisolone succinate sodium  (Synonyms: Methylprednisolone hydrogen succinate sodium)

Cat. No.: HY-B1060 Purity: 99.04%
COA Handling Instructions

Methylprednisolone succinate (Methylprednisolone hydrogen succinate) sodium, a glucocorticoid, is a immunosuppressive agent with anti-inflammatory effects.

For research use only. We do not sell to patients.

Methylprednisolone succinate sodium Chemical Structure

Methylprednisolone succinate sodium Chemical Structure

CAS No. : 2375-03-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 55 In-stock
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid
5 mg USD 32 In-stock
10 mg USD 48 In-stock
25 mg USD 91 In-stock
50 mg USD 136 In-stock
100 mg USD 200 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Methylprednisolone succinate sodium:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Methylprednisolone succinate (Methylprednisolone hydrogen succinate) sodium, a glucocorticoid, is a immunosuppressive agent with anti-inflammatory effects[1].

In Vitro

Methylprednisolone succinate sodium (1-400 μg; 2 days) inhibits cell growth of human glioblastomas in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Administration of Methylprednisolone succinate sodium post- intracranial haemorrhage (ICH) significantly reduces permeability of the blood-brain barrier (BBB) and brain oedema and upregulated expression of ZO-1 and Occludin. Methylprednisolone succinate sodium inhibits inflammatory responses, including reducing proinflammatory cytokines (IL-1β, TNF-α), suppressing infiltration of neutrophils and activation of microglia. In addition, Methylprednisolone succinate sodium reduces neuronal apoptosis through increasing Bcl-2 expression and reducing Bax expression[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57/BL mouse (6-8 weeks old, 20-23 g) injected with collagenase[1]
Dosage: 30 mg/kg
Administration: ip; at 2, 24 and 48 h after ICH
Result: Significantly reduced permeability of the BBB and brain oedema and upregulated expression of ZO-1 and Occludin.
Clinical Trial
Molecular Weight

496.53

Formula

C26H33NaO8

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](C(COC(CCC(O[Na])=O)=O)=O)(O)CC[C@@]1([H])[C@]3([H])C[C@H](C)C4=CC(C=C[C@]4(C)[C@@]3([H])[C@@H](O)C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (100.70 mM; Need ultrasonic)

DMSO : ≥ 25 mg/mL (50.35 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0140 mL 10.0699 mL 20.1398 mL
5 mM 0.4028 mL 2.0140 mL 4.0280 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.19 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.04%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.0140 mL 10.0699 mL 20.1398 mL 50.3494 mL
5 mM 0.4028 mL 2.0140 mL 4.0280 mL 10.0699 mL
10 mM 0.2014 mL 1.0070 mL 2.0140 mL 5.0349 mL
15 mM 0.1343 mL 0.6713 mL 1.3427 mL 3.3566 mL
20 mM 0.1007 mL 0.5035 mL 1.0070 mL 2.5175 mL
25 mM 0.0806 mL 0.4028 mL 0.8056 mL 2.0140 mL
30 mM 0.0671 mL 0.3357 mL 0.6713 mL 1.6783 mL
40 mM 0.0503 mL 0.2517 mL 0.5035 mL 1.2587 mL
50 mM 0.0403 mL 0.2014 mL 0.4028 mL 1.0070 mL
H2O 60 mM 0.0336 mL 0.1678 mL 0.3357 mL 0.8392 mL
80 mM 0.0252 mL 0.1259 mL 0.2517 mL 0.6294 mL
100 mM 0.0201 mL 0.1007 mL 0.2014 mL 0.5035 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Methylprednisolone succinate sodium
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HY-B1060
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