7-Cyano-7-deazaguanine
Based on 1 Customer Validation
7-Cyano-7-deazaguanine is a key purine precursor molecule in the 7-deazaguanine pathway. 7-Cyano-7-deazaguanine acts as a substrate for transglycosylases to participate in base exchange reactions, replacing native guanine in nucleic acids to generate 7-deazaguanine-modified DNA or tRNA; it can be catalyzed by QueC/ToyM to form the 7-amido-7-deazaguanine (ADG) intermediate for further involvement in subsequent biosynthesis. 7-Cyano-7-deazaguanine is applicable to studies related to translation regulation.
For research use only. We do not sell to patients.
- CAS No.: 69205-79-4
- Formula: C7H5N5O
- Molecular Weight:175.15
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
7-Cyano-7-deazaguanine (preQ0) serves as a substrate for a variety of purified enzymes: Bacillus subtilis QueC generates it from CDG relying on K163 and R204; Pyrococcus arcTGT inserts it into archaeal tRNA; phage DpdA1 integrates it into specific DNA sequences; Salmonella enterica serovar Montevideo DpdC converts preQ0-modified DNA into dADG-modified DNA; and it can be transported across the inner membrane by recombinant Escherichia coli QPTR[1].
7‑Cyano‑7‑deazaguanine (preQ0) is incorporated into pUC19 DNA as dPreQ0 and subsequently converted to dADG by DpdC from Salmonella enterica serovar Montevideo[3].
7‑Cyano‑7‑deazaguanine (preQ0) is the final product of the two-step conversion of CDG to ADG catalyzed by ATP-dependent ToyM, with a half-life of approximately 4 min for the formation of this product from ADG[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 69205-79-4
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Appearance Solid
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Molecular Weight 175.15
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Formula C7H5N5O
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SMILES
N#CC1=CNC(N=C(N)N2)=C1C2=O
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Structure Classification
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Initial Source
Escherichia coli
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 6.25 mg/mL (35.68 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.7094 mL | 28.5470 mL | 57.0939 mL | 142.7348 mL |
| 5 mM | 1.1419 mL | 5.7094 mL | 11.4188 mL | 28.5470 mL | |
| 10 mM | 0.5709 mL | 2.8547 mL | 5.7094 mL | 14.2735 mL | |
| 15 mM | 0.3806 mL | 1.9031 mL | 3.8063 mL | 9.5157 mL | |
| 20 mM | 0.2855 mL | 1.4273 mL | 2.8547 mL | 7.1367 mL | |
| 25 mM | 0.2284 mL | 1.1419 mL | 2.2838 mL | 5.7094 mL | |
| 30 mM | 0.1903 mL | 0.9516 mL | 1.9031 mL | 4.7578 mL |
7-Cyano-7-deazaguanine Related Classifications
HY-W003845 Related Classifications
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