Antennapedia Peptide
Based on 1 Customer Validation
Antennapedia Peptide (Penetratin peptide) is a 16 amino acid peptide, originally derived from the 60 amino acid long homeodomain of the Drosophila transcription factor Antennapedia and is a member of the family of cell-penetrating peptides.
For research use only. We do not sell to patients.
- Purity : 98.35%
- CAS No.: 188842-14-0
- Formula: C104H168N34O20S
- Molecular Weight:2246.73
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
>100 μM
Compound: Penetratin
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Cytostatic activity against human MCF7 cells preincubated for 3 hrs followed by serum treatment for 72 hrs measured on day 4 by MTT assay
Cytostatic activity against human MCF7 cells preincubated for 3 hrs followed by serum treatment for 72 hrs measured on day 4 by MTT assay
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[PMID: 27031212] |
| MDA-MB-231 | IC50 |
>100 μM
Compound: Penetratin
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Cytostatic activity against human MDA-MB-231 cells preincubated for 3 hrs followed by serum treatment for 72 hrs measured on day 4 by MTT assay
Cytostatic activity against human MDA-MB-231 cells preincubated for 3 hrs followed by serum treatment for 72 hrs measured on day 4 by MTT assay
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[PMID: 27031212] |
In Vitro
Antennapedia (Antp)-SMCC-cytochrome c conjugate (5 μg/mL) activates caspase-dependent apoptosis of HeLa cells. The Antp-SMCC-cytochrome c conjugate reduces the clonogenic survival of Hela cells, and inhibits clonogenic survival[1]. ANTP-SmacN7 fusion protein can transduce and accumulate in cells, while SmacN7 alone cannot. ANTP-SmacN7 fusion proteins have a radiation-sensitising effect on EC109 cells. ANTP-SmacN7 combined with radiation does not promote further increases in caspase-3 protein expression, it increases the levels of cleaved caspase-3[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 188842-14-0
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Appearance Solid
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Molecular Weight 2246.73
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Formula C104H168N34O20S
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Color White to off-white
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Synonyms
Penetratin peptide
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Sequence
Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys
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Sequence Shortening
RQIKIWFQNRRMKWKK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (44.51 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Nuclear Protein Extraction (High-Salt/Hypotonic Fractionation)
The high-salt/hypotonic fractionation method for nuclear protein extraction is based on the differential solubility of cellular components. Cytoplasmic proteins are extracted first using a hypotonic buffer that causes cell swelling and membrane rupture, followed by centrifugation to separate the cytoplasmic supernatant from the nuclear pellet. The nuclear pellet is then subjected to high-salt extraction (e. g. , 0. 4 M (NH4)2SO4 or 1 M NaCl) to solubilize tightly bound nuclear matrix proteins, including transcription factors, histones, and structural proteins associated with chromatin and the nuclear scaffold. This approach allows for the isolation of both soluble cytoplasmic proteins and salt-resistant nuclear proteins while minimizing cross-contamination.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Imesch P, et al. Conjugates of cytochrome c and antennapedia peptide activate apoptosis and inhibit proliferation of HeLa cancer cells. Exp Ther Med. 2013 Sep;6(3):786-790. Epub 2013 Jul 4. [Content Brief]
[2]. Du LQ, et al. Radiation-sensitising effects of antennapedia proteins (ANTP)-SmacN7 on tumour cells. Int J Mol Sci. 2013 Dec 11;14(12):24087-96. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4451 mL | 2.2255 mL | 4.4509 mL | 11.1273 mL |
| 5 mM | 0.0890 mL | 0.4451 mL | 0.8902 mL | 2.2255 mL | |
| 10 mM | 0.0445 mL | 0.2225 mL | 0.4451 mL | 1.1127 mL | |
| 15 mM | 0.0297 mL | 0.1484 mL | 0.2967 mL | 0.7418 mL | |
| 20 mM | 0.0223 mL | 0.1113 mL | 0.2225 mL | 0.5564 mL | |
| 25 mM | 0.0178 mL | 0.0890 mL | 0.1780 mL | 0.4451 mL | |
| 30 mM | 0.0148 mL | 0.0742 mL | 0.1484 mL | 0.3709 mL | |
| 40 mM | 0.0111 mL | 0.0556 mL | 0.1113 mL | 0.2782 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Antennapedia Peptide Related Classifications
HY-P0307 Related Classifications
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