CARM1-IN-1 hydrochloride
Based on 4 publication(s) in Google Scholar
CARM1-IN-1 (compound 7g) hydrochloride is a highly potent and selective inhibitor of CARM1 (IC50=8.6 μM, CARM1/PABP1), with low inhibitory activity against PRMT1 and SET7 (IC50 >667 μM). CARM1-IN-1 hydrochloride inhibits the methylation activity of CARM1 and the methylation levels of different substrates, such as PABP1, CA150, SmB, and H3. CARM1-IN-1 hydrochloride also inhibits the promoter activity of prostate-specific antigen (PSA) without significant cytotoxicity.
For research use only. We do not sell to patients.
- Purity: 95.17%
- CAS No.: 2070018-31-2
- Formula: C26H22Br2ClNO3
- Molecular Weight:591.72
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Storage:
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
![His-[D-2-ME-Trp]-Ala](https://file.medchemexpress.com/product_pic/hy-p1460.gif)
Publications Citing Use of MedChemExpress (MCE) CARM1-IN-1 hydrochloride
MoreAll Histone Methyltransferase Isoforms
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Biological Activity
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PRMT4 |
CARM1-IN-1 (compound 7g) hydrochloride (100 μM; 90 min) inhibits CARM1-mediated methylation of different substrates (PABP1, CA150, SmB, and H3), such as Flag-PABP1 methylation, without inhibitory activity against HKMTs[1].
CARM1-IN-1 (compound 7g) hydrochloride (8 μM; 42-44 h) inhibits PSA promoter activity in luciferase assay in LNCaP cells, without affecting cell viability[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PCR LNCa cells
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Concentration:1 μM, 2 μM, 4 μM, 6 μM, 8 μM
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Incubation Time:42-48 h
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Result:Showed a significant dose-dependent reduction of the PSA promoter activity, at concentration that did not affect cell viability.
Chemical Information
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CAS No. 2070018-31-2
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Appearance Solid
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Molecular Weight 591.72
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Formula C26H22Br2ClNO3
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Color Light yellow to yellow
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SMILES
[H]Cl.O=C1/C(CN(CC2=CC=CC=C2)C/C1=C\C3=CC=C(O)C(Br)=C3)=C/C4=CC=C(O)C(Br)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (4)
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Journal Impact Factor
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Most Recent
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Sci Bull (Beijing)
TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis. [Abstract]2019 Jul 30;64(14):986-997. PMID: 36659810 -
Theriogenology
Unveiling the effects of regulating endogenous growth factors and apoptosis via DGCR8 on yak blastocyst formation. [Abstract]2026 Feb 1:251:117750. PMID: 41240745 -
Theriogenology
Paraspeckles / CARM1 mediates the regulation of OEVs on cell differentiation during in vitro embryonic development of yak. [Abstract]2024 Jul 15:223:74-88. PMID: 38692037 -
Solvent & Solubility
DMSO : 250 mg/mL (422.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.22 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6900 mL | 8.4499 mL | 16.8999 mL | 42.2497 mL |
| 5 mM | 0.3380 mL | 1.6900 mL | 3.3800 mL | 8.4499 mL | |
| 10 mM | 0.1690 mL | 0.8450 mL | 1.6900 mL | 4.2250 mL | |
| 15 mM | 0.1127 mL | 0.5633 mL | 1.1267 mL | 2.8166 mL | |
| 20 mM | 0.0845 mL | 0.4225 mL | 0.8450 mL | 2.1125 mL | |
| 25 mM | 0.0676 mL | 0.3380 mL | 0.6760 mL | 1.6900 mL | |
| 30 mM | 0.0563 mL | 0.2817 mL | 0.5633 mL | 1.4083 mL | |
| 40 mM | 0.0422 mL | 0.2112 mL | 0.4225 mL | 1.0562 mL | |
| 50 mM | 0.0338 mL | 0.1690 mL | 0.3380 mL | 0.8450 mL | |
| 60 mM | 0.0282 mL | 0.1408 mL | 0.2817 mL | 0.7042 mL | |
| 80 mM | 0.0211 mL | 0.1056 mL | 0.2112 mL | 0.5281 mL | |
| 100 mM | 0.0169 mL | 0.0845 mL | 0.1690 mL | 0.4225 mL |
CARM1-IN-1 hydrochloride Related Classifications
HY-12759A Related Classifications
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