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Docosahexaenoic Acid methyl ester (Synonyms: all cis-DHA methyl ester; Methyl docosahexaenoate)

Cat. No.: HY-101541 Purity: >95.0%
Handling Instructions

Docosahexaenoic Acid methyl ester is a methylated docosahexaenoic acid analog which can be intercalated into membrane phospholipids without being oxidized or hydrolyzed.

For research use only. We do not sell to patients.

Docosahexaenoic Acid methyl ester Chemical Structure

Docosahexaenoic Acid methyl ester Chemical Structure

CAS No. : 2566-90-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Docosahexaenoic Acid methyl ester is a methylated docosahexaenoic acid analog which can be intercalated into membrane phospholipids without being oxidized or hydrolyzed.

In Vitro

Sharp wave (SPW) incidence relative to baseline appears to decrease following Docosahexaenoic Acid methyl ester (DHA-Me) application[1]. There is no generation of a new protein band when bovine serum albumin (BSA) is exposed to the Fe2+ and ascorbic acid (AsA) mixed-function oxidation system in the absence of Docosahexaenoic Acid methyl ester (DHA). However, the high-molecular-weight protein band is observed after only 24 h when BSA is incubated with DHA. Incubation of BSA with 1.0 mM DHA leads to a substantial increase in protein carbonyl content and the addition of oxygen radical scavengers leads to a substantial decrease in protein carbonyl content[2].

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (72.99 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9196 mL 14.5981 mL 29.1962 mL
5 mM 0.5839 mL 2.9196 mL 5.8392 mL
10 mM 0.2920 mL 1.4598 mL 2.9196 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution

References
Kinase Assay
[2]

Protein (1 mg/mL) is incubated with Docosahexaenoic Acid methyl ester (DHA) (1.0 mM) in 50 mM HEPES buffer (pH 7.4) containing 0.2% (w/v) of Tween 20 at 37°C. Mannitol, histidine, sodium benzoate, or KI is added at final concentration of 0.5 M, 0.2 M, 0.5 M, or 0.1 M, respectively. Reactive oxygen species are generated by addition of FeSO4 (1 μM) and ascorbic acid (AsA, 20 μM)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

342.51

Formula

C₂₃H₃₄O₂

CAS No.

2566-90-7

SMILES

CC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CCC(OC)=O

Storage
Pure form -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Docosahexaenoic Acid methyl ester
Cat. No.:
HY-101541
Quantity:

Docosahexaenoic Acid methyl ester

Cat. No.: HY-101541