1. Cell Cycle/DNA Damage
    Cytoskeleton
  2. Microtubule/Tubulin

Epothilone A (Synonyms: Epo A)

Cat. No.: HY-13503 Purity: 99.05%
Handling Instructions

Epothilone A is a competitive inhibitor of the binding of [3H] paclitaxel to tubulin polymers, with a Ki of 0.6-1.4 μM.

For research use only. We do not sell to patients.

Epothilone A Chemical Structure

Epothilone A Chemical Structure

CAS No. : 152044-53-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 109 In-stock
Estimated Time of Arrival: December 31
1 mg USD 50 In-stock
Estimated Time of Arrival: December 31
5 mg USD 100 In-stock
Estimated Time of Arrival: December 31
10 mg USD 160 In-stock
Estimated Time of Arrival: December 31
25 mg USD 320 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

Epothilone A is a competitive inhibitor of the binding of [3H] paclitaxel to tubulin polymers, with a Ki of 0.6-1.4 μM.

In Vitro

Epothilone A is a competitive inhibitor of the binding of [3H] paclitaxel to tubulin polymers. The apparent Ki value for Epothilone A is 1.4 μM by Hanes analysis and 0.6 μM by Dixon analysis[1]. Epothilone A, is noted to be highly cytotoxic (IC50=0.05 μM) in vitro when applied to the human T-24 bladder carcinoma cell line. The binding affinity of Epothilone A to tubulin is of the same order of magnitude as the binding affinity of paclitaxel to tubulin based on competition assays. The IC50 for displacement of 100 nM of (3H) paclitaxel from the tubulin binding site is 3.6 μM for paclitaxel, 2.3 μM for Epothilone A, and 3.3 μM for patupilone[2].

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (253.21 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0257 mL 10.1284 mL 20.2569 mL
5 mM 0.4051 mL 2.0257 mL 4.0514 mL
10 mM 0.2026 mL 1.0128 mL 2.0257 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

References
Molecular Weight

493.66

Formula

C₂₆H₃₉NO₆S

CAS No.

152044-53-6

SMILES

O=C(C[[email protected]](O)C1(C)C)O[[email protected]](/C(C)=C/C2=CSC(C)=N2)C[[email protected]]3([H])O[[email protected]]3([H])CCC[[email protected]](C)[[email protected]](O)[[email protected]@H](C)C1=O

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Epothilone A
Cat. No.:
HY-13503
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Epothilone A

Cat. No.: HY-13503