Jujuboside A
Based on 4 publication(s) in Google Scholar
Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 55466-04-1
- Formula: C58H94O26
- Molecular Weight:1207.35
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
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Publications Citing Use of MedChemExpress (MCE) Jujuboside A
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Customer Validation & Images
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WB
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Cell Proliferation/Viability Assay
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Apoptosis Analysis
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IF
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Flow Cytometry
Biological Activity
Jujuboside A at the low dose of 41 μM (about 0.05 g/L) induces significant increase of GABA(A) receptor α1, α5, β2 subunit mRNAs in both 24 and 72h treatments. Jujuboside A at the high dose of 82 μM (about 0.1 g/L) significantly increases GABA(A) receptor α1, α5 subunit mRNA levels and decreases β2 subunit mRNA level at 24h treatment, and decreases GABA(A) receptor subunit α1, β2 mRNAs expression at 72h treatment[1]. Jujuboside A pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO. Jujuboside A could accelerate the phosphorylation of PI3K, Akt, and mTOR. Jujuboside A could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 55466-04-1
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Appearance Solid
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Molecular Weight 1207.35
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Formula C58H94O26
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Color White to off-white
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SMILES
C[C@]([C@]1([H])CC2)(CC[C@]3([H])[C@@]1(CC[C@H](O[C@@](OC[C@H](O)[C@@H]4O[C@@](O[C@H](CO[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO)[C@@H](O)[C@@H]6O)([H])[C@@H]6O[C@@](OC[C@@H](O)[C@@H]7O)([H])[C@@H]7O)([H])[C@@H]4O[C@@](O[C@@H](C)[C@H](O)[C@H]8O)([H])[C@@H]8O)C3(C)C)C)[C@@]9(CO%10)[C@@]2([H])[C@]([C@]%11(O)C)([H])[C@@]%10(O[C@@H](/C=C(C)/C)C%11)C9
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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Cancer Biol Ther
Jujuboside A induces bladder cancer cell apoptosis by inhibiting ATP1A2-mediated mitochondrial energy metabolism regulation. [Abstract]2026 Dec 31;27(1):2615418. PMID: 41533486
Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]
T24 cells were treated with different concentrations of Jujuboside A (JuA) (12.5, 25, and 50 μmol/L) for 48 h. Changes in relative ATP1A2 protein levels were subsequently assessed via western blotting.
Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]
After treating T24 cells with different concentrations of Jujuboside A (JuA) (6.125, 12.5, 25, 50, and 100 μmol/L) for 48 h, changes in cell viability were analyzed via CCK8 assay.
Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]
After treatment with Jujuboside A (JuA) (50 μmol/L) and overexpression plasmid transfection, changes in the cell apoptosis ratio were assessed via flow cytometry with FITC/PI double staining.
Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]
After treating with 50 μmol/L of Jujuboside A (JuA) for 48 h, transfecting with the ATP1A2 overexpression plasmid, and culturing for another 48 h, changes in the mitochondrial membrane potential of T24 cells were assessed via a JC-1 mitochondrial membrane potential fluorescence probe assay.
Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]
After treatment with Jujuboside A (JuA) (50 μmol/L) and overexpression plasmid transfection, changes in the intracellular ROS levels in T24 cells were assessed via flow cytometry.
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Biochim Biophys Acta Mol Basis Dis
2024 Sep 3:167496. PMID: 39237046 -
Med Oncol
FAT4 activation inhibits epithelial-mesenchymal transition (EMT) by promoting autophagy in H2228/Cer cells. [Abstract]2022 Dec 28;40(1):64. PMID: 36576661 -
Solvent & Solubility
DMSO : 100 mg/mL (82.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (41.41 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (41.41 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
After 7 days in culture in vitro, cells are exposed continuously for the next 24 or 72 h to the culture medium with either Jujuboside A or diazepam, or neither of them. Vehicle is added for the control group; 10 μM diazepam is added for the diazepam group; Jujuboside A 82 μM (about 0.1 g/L) and 41 μM (about 0.05 g/L) are added for the JuA-H (high dose Jujuboside A) and the JuA-L (low dose Jujuboside A) groups respectively. Total RNA is isolated from cells for further analysis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice: Cognitive impairment of mice is induced by ICV injection of Aβ1-42. Then mice are given intracerebroventricular (ICV) injection of Jujuboside A (0.02 and 0.2 mg/kg) for five consecutive days. Y-maze, active avoidance and Morris water maze tests are performed on mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. You ZL, et al. Effects on the expression of GABAA receptor subunits by jujuboside A treatment in rathippocampal neurons. J Ethnopharmacol. 2010 Mar 24;128(2):419-23. [Content Brief]
[2]. Han D, et al. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway. Evid Based Complement Alternat Med. 2016;2016:9593716. [Content Brief]
[3]. Cao JX, et al. Hypnotic effect of jujubosides from Semen Ziziphi Spinosae. J Ethnopharmacol. 2010 Jul 6;130(1):163-6. [Content Brief]
[4]. Liu Z, et al. Jujuboside A, a neuroprotective agent from semen Ziziphi Spinosae ameliorates behavioral disorders of the dementia mouse model induced by Aβ 1-42. Eur J Pharmacol. 2014 Sep 5;738:206-13. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.8283 mL | 4.1413 mL | 8.2826 mL | 20.7065 mL |
| 5 mM | 0.1657 mL | 0.8283 mL | 1.6565 mL | 4.1413 mL | |
| 10 mM | 0.0828 mL | 0.4141 mL | 0.8283 mL | 2.0707 mL | |
| 15 mM | 0.0552 mL | 0.2761 mL | 0.5522 mL | 1.3804 mL | |
| 20 mM | 0.0414 mL | 0.2071 mL | 0.4141 mL | 1.0353 mL | |
| 25 mM | 0.0331 mL | 0.1657 mL | 0.3313 mL | 0.8283 mL | |
| 30 mM | 0.0276 mL | 0.1380 mL | 0.2761 mL | 0.6902 mL | |
| 40 mM | 0.0207 mL | 0.1035 mL | 0.2071 mL | 0.5177 mL | |
| DMSO | 50 mM | 0.0166 mL | 0.0828 mL | 0.1657 mL | 0.4141 mL |
| 60 mM | 0.0138 mL | 0.0690 mL | 0.1380 mL | 0.3451 mL | |
| 80 mM | 0.0104 mL | 0.0518 mL | 0.1035 mL | 0.2588 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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