MLCK inhibitor peptide 18
Based on 6 publication(s) in Google Scholar
MLCK inhibitor peptide 18 is a myosin light chain kinase (MLCK) inhibitor with an IC50 of 50 nM, and inhibits CaM kinase II only at 4000-fold higher concentrations.
For research use only. We do not sell to patients.
- Purity : 98.58%
- CAS No.: 224579-74-2
- Formula: C60H105N23O11
- Molecular Weight:1324.62
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) MLCK inhibitor peptide 18
More- Bioact Mater. 2025 Nov 25:57:646-659. [Abstract]
- Phytomedicine. 2024 Dec:135:156111. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2021;11(3):683-696. [Abstract]
- J Cell Sci. 2022 Jul 1;135(13):jcs259692. [Abstract]
- Life Sci Alliance. 2024 Aug 9;7(11):e202402885. [Abstract]
- Biomed Pharmacother. 2025 May:186:118025. [Abstract]
Biological Activity
Description
IC50 & Target
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CaMK II |
In Vitro
MLCK inhibitor peptide 18 has a selective effect on peptide substrate utilization by MLCK, does not interfere with kinase activation by CaM, does not have significant inhibitory activity with the closely related CaMPKII, and does not inhibit PKA[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 224579-74-2
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Appearance Solid
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Molecular Weight 1324.62
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Formula C60H105N23O11
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Color White to off-white
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Sequence
Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH2
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Sequence Shortening
RKKYKYRRK-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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Bioact Mater
Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges. [Abstract]2025 Nov 25:57:646-659. PMID: 41377894 -
Phytomedicine
Swiprosin-1 participates in the berberine-regulated AMPK/MLCK pathway to attenuate colitis-induced tight junction damage. [Abstract]2024 Dec:135:156111. PMID: 39369569 -
Cell Mol Gastroenterol Hepatol
2021;11(3):683-696. PMID: 33075564 -
J Cell Sci
2022 Jul 1;135(13):jcs259692. PMID: 35665815 -
Life Sci Alliance
JAK inhibition with tofacitinib rapidly increases contractile force in human skeletal muscle. [Abstract]2024 Aug 9;7(11):e202402885. PMID: 39122555 -
Biomed Pharmacother
Homoharringtonine (omacetaxine mepesuccinate) limits the angiogenic capacity of endothelial cells and reorganises filamentous actin. [Abstract]2025 May:186:118025. PMID: 40184838
Solvent & Solubility
In Vitro:
H2O : 100 mg/mL (75.49 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (75.49 mM); Clear solution; Need ultrasonic
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.7549 mL | 3.7747 mL | 7.5493 mL | 18.8733 mL |
| 5 mM | 0.1510 mL | 0.7549 mL | 1.5099 mL | 3.7747 mL | |
| 10 mM | 0.0755 mL | 0.3775 mL | 0.7549 mL | 1.8873 mL | |
| 15 mM | 0.0503 mL | 0.2516 mL | 0.5033 mL | 1.2582 mL | |
| 20 mM | 0.0377 mL | 0.1887 mL | 0.3775 mL | 0.9437 mL | |
| 25 mM | 0.0302 mL | 0.1510 mL | 0.3020 mL | 0.7549 mL | |
| 30 mM | 0.0252 mL | 0.1258 mL | 0.2516 mL | 0.6291 mL | |
| 40 mM | 0.0189 mL | 0.0944 mL | 0.1887 mL | 0.4718 mL | |
| 50 mM | 0.0151 mL | 0.0755 mL | 0.1510 mL | 0.3775 mL | |
| 60 mM | 0.0126 mL | 0.0629 mL | 0.1258 mL | 0.3146 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
MLCK inhibitor peptide 18 Related Classifications
HY-P1029 Related Classifications
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