Orexin 2 Receptor Agonist
Based on 1 Customer Validation
Orexin 2 Receptor Agonist is a potent selective OX2R agonist with an EC50 of 23 nM.
For research use only. We do not sell to patients.
- Purity: 99.59%
- CAS No.: 1796565-52-0
- Formula: C32H34N4O5S
- Molecular Weight:586.70
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Orexin Receptor (OX Receptor) Isoforms
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Biological Activity
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OX2 Receptor |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO-K1 | EC50 |
0.023 μM
Compound: 26
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Agonist activity at human OX2R expressed in CHOK1 cells by NFAT-response luciferase reporter gene assay
Agonist activity at human OX2R expressed in CHOK1 cells by NFAT-response luciferase reporter gene assay
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[PMID: 26267383] |
| CHO-K1 | EC50 |
1.616 μM
Compound: 26
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Agonist activity at human OX1R expressed in CHOK1 cells by calcium mobilization assay
Agonist activity at human OX1R expressed in CHOK1 cells by calcium mobilization assay
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[PMID: 26267383] |
Orexin 2 Receptor Agonist replaces Orexin-A to bind to hOX1R and hOX2R in a concentration-dependent manner in CHO cells overexpressing hOX1R and HEK-293 cells overexpressing hOX2R with Ki values of 0.14 μM and 0.77 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1796565-52-0
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Appearance Solid
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Molecular Weight 586.70
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Formula C32H34N4O5S
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Color White to off-white
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SMILES
O=C(C1=CC=CC(C)=C1)NCCNC2=CC(NS(C3=C(OC)C=CC(C4=CC(C(N(C)C)=O)=CC=C4)=C3)(=O)=O)=CC=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 32 mg/mL (54.54 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.26 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7044 mL | 8.5222 mL | 17.0445 mL | 42.6112 mL |
| 5 mM | 0.3409 mL | 1.7044 mL | 3.4089 mL | 8.5222 mL | |
| 10 mM | 0.1704 mL | 0.8522 mL | 1.7044 mL | 4.2611 mL | |
| 15 mM | 0.1136 mL | 0.5681 mL | 1.1363 mL | 2.8407 mL | |
| 20 mM | 0.0852 mL | 0.4261 mL | 0.8522 mL | 2.1306 mL | |
| 25 mM | 0.0682 mL | 0.3409 mL | 0.6818 mL | 1.7044 mL | |
| 30 mM | 0.0568 mL | 0.2841 mL | 0.5681 mL | 1.4204 mL | |
| 40 mM | 0.0426 mL | 0.2131 mL | 0.4261 mL | 1.0653 mL | |
| 50 mM | 0.0341 mL | 0.1704 mL | 0.3409 mL | 0.8522 mL |
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