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  4. PL553

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.

For research use only. We do not sell to patients.

PL553

PL553 Chemical Structure

CAS No. : 1456872-74-4

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Based on 1 publication(s) in Google Scholar

PL553 Related Antibodies

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Description

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.

In Vitro

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λmax) of 210 nm and maximum emission (λem) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PL553 Related Antibodies
Molecular Weight

508.49

Formula

C28H23F3N2O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C2C=CC=CC2=C1)[C@@H](N)CC3=CC=C(C(C4=CC=CC=C4)=O)C=C3.O=C(O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (393.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9666 mL 9.8330 mL 19.6661 mL
5 mM 0.3933 mL 1.9666 mL 3.9332 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Kinase Assay
[1]

The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9666 mL 9.8330 mL 19.6661 mL 49.1652 mL
5 mM 0.3933 mL 1.9666 mL 3.9332 mL 9.8330 mL
10 mM 0.1967 mL 0.9833 mL 1.9666 mL 4.9165 mL
15 mM 0.1311 mL 0.6555 mL 1.3111 mL 3.2777 mL
20 mM 0.0983 mL 0.4917 mL 0.9833 mL 2.4583 mL
25 mM 0.0787 mL 0.3933 mL 0.7866 mL 1.9666 mL
30 mM 0.0656 mL 0.3278 mL 0.6555 mL 1.6388 mL
40 mM 0.0492 mL 0.2458 mL 0.4917 mL 1.2291 mL
50 mM 0.0393 mL 0.1967 mL 0.3933 mL 0.9833 mL
60 mM 0.0328 mL 0.1639 mL 0.3278 mL 0.8194 mL
80 mM 0.0246 mL 0.1229 mL 0.2458 mL 0.6146 mL
100 mM 0.0197 mL 0.0983 mL 0.1967 mL 0.4917 mL
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PL553 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PL5531456872-74-4PL 553PL-553Fluorescent DyeInhibitorinhibitorinhibit

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