1. Dye Reagents

PL553 

Cat. No.: HY-U00452
Handling Instructions

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.

For research use only. We do not sell to patients.

PL553 Chemical Structure

PL553 Chemical Structure

CAS No. : 1456872-74-4

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.

In Vitro

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λmax) of 210 nm and maximum emission (λem) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H[1].

References
Kinase Assay
[1]

The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

508.49

Formula

C₂₈H₂₃F₃N₂O₄

CAS No.

1456872-74-4

SMILES

O=C(NC1=CC=C2C=CC=CC2=C1)[[email protected]@H](N)CC3=CC=C(C(C4=CC=CC=C4)=O)C=C3.O=C(O)C(F)(F)F

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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PL553
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PL553

Cat. No.: HY-U00452