PL553
Based on 1 Customer Validation
PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.
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- Purity: 99.78%
- CAS No.: 1456872-74-4
- 화학식: C28H23F3N2O4
- 분자량:508.49
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λmax) of 210 nm and maximum emission (λem) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1456872-74-4
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Appearance Solid
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분자량 508.49
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화학식 C28H23F3N2O4
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Color White to off-white
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SMILES
O=C(NC1=CC=C2C=CC=CC2=C1)[C@@H](N)CC3=CC=C(C(C4=CC=CC=C4)=O)C=C3.O=C(O)C(F)(F)F
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 200 mg/mL (393.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9666 mL | 9.8330 mL | 19.6661 mL | 49.1652 mL |
| 5 mM | 0.3933 mL | 1.9666 mL | 3.9332 mL | 9.8330 mL | |
| 10 mM | 0.1967 mL | 0.9833 mL | 1.9666 mL | 4.9165 mL | |
| 15 mM | 0.1311 mL | 0.6555 mL | 1.3111 mL | 3.2777 mL | |
| 20 mM | 0.0983 mL | 0.4917 mL | 0.9833 mL | 2.4583 mL | |
| 25 mM | 0.0787 mL | 0.3933 mL | 0.7866 mL | 1.9666 mL | |
| 30 mM | 0.0656 mL | 0.3278 mL | 0.6555 mL | 1.6388 mL | |
| 40 mM | 0.0492 mL | 0.2458 mL | 0.4917 mL | 1.2291 mL | |
| 50 mM | 0.0393 mL | 0.1967 mL | 0.3933 mL | 0.9833 mL | |
| 60 mM | 0.0328 mL | 0.1639 mL | 0.3278 mL | 0.8194 mL | |
| 80 mM | 0.0246 mL | 0.1229 mL | 0.2458 mL | 0.6146 mL | |
| 100 mM | 0.0197 mL | 0.0983 mL | 0.1967 mL | 0.4917 mL |
PL553 Related Classifications
HY-U00452 Related Classifications
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