DL-O-Phosphoserine 

DL-O-Phosphoserine is a normal metabolite in human biofluids and is a mixture of phosphoserine with two stereochemical structures (D-type and L-type O-phospho-serine). O-phospho-L-serine is a competitive inhibitor of serine racemase and phagocytosis. O-phospho-L-serine inhibits the enzymatic reaction by competing with the active site of serine racemase, thereby affecting the production of D-serine. O-phospho-L-serine also mimics the phosphatidylserine head group, binds to the phosphatidylserine receptor, interferes with phagocytic signal transduction, and partially blocks the phagocytosis of apoptotic cells by cells. O-phospho-L-serin can be used in the research of retinal regeneration, bone repair, and insect growth and development^[1][2].

O-phospho-L-serin shows inhibitory activity agasint serine racemase in a concentration-dependent manner, with the enzyme reaction inhibition rate of approximately 20% at 2 mM concentration^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

O-phospho-L-serin can be used in retinal research to explore the regulatory mechanism of Müller glial cell proliferation and photoreceptor regeneration; in bone repair research, to evaluate the effects on bone cement performance and bone healing; in insect research, to identify the role of D-serine in insect growth and development. In the zebrafish light-damaged retinal model experiment, O-phospho-L-serin (20 mM, 0.5 μL; iv; injected before light treatment; single dose) can significantly reduce the number of proliferating cell nuclear antigen (PCNA)-positive Müller glial cells 51 hours after light damage, and inhibit the regeneration of cones in the light-damaged retina, but has no significant effect on the number of light-induced photoreceptor cell death^[1].
In a miniature pig mandibular bone defect model experiment, a modified bone cement prepared by surgical implantation of a mixture of O-phospho-L-serin (Biozement D (2 g)-collagen-I (2.5%)-phosphoserine (50 mg)) was administered. Compared with the unmodified bone cement, the treated group had a higher absorption rate and bone regeneration rate, which effectively promoted bone healing^[2].
In a silkworm growth and development experiment, O-phospho-L-serin (20 mM, 1 mL; added to feed; daily administration, from first to fifth instar larvae) delayed the development of silkworm larvae by about 6 days, significantly reduced the survival rate of larvae, and reduced the D-serine level in larvae until the cocooning stage compared with the control group^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

185.07

C₃H₈NO₆P

17885-08-4

Solid

White to off-white

OP(OCC(C(O)=O)N)(O)=O

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Bailey TJ, et al. The inhibitor of phagocytosis, O-phospho-L-serine, suppresses Müller glia proliferation and cone cell regeneration in the light-damaged zebrafish retina. Exp Eye Res. 2010 Nov;91(5):601-12.  [Content Brief]

  [2]. Mai R, Lux R, Proff P, Lauer G, Pradel W, Leonhardt H, Reinstorf A, Gelinsky M, Jung R, Eckelt U, Gedrange T, Stadlinger B. O-phospho-L-serine: a modulator of bone healing in calcium-phosphate cements. Biomed Tech (Berl). 2008 Oct;53(5):229-33.  [Content Brief]