1. Anti-infection
  2. HCV
  3. TMC647055 Choline salt

TMC647055 (Choline salt) is a potent nonnucleoside NS5B polymerase inhibitor of HCV replication. TMC647055 Choline salt has potent HCV combine activity with an IC50 value of 82 nM. TMC647055 Choline salt can be used for the research of Hepatitis C virus (HCV).

For research use only. We do not sell to patients.

TMC647055 Choline salt Chemical Structure

TMC647055 Choline salt Chemical Structure

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 432 In-stock
Solution
10 mM * 1 mL in DMSO USD 432 In-stock
Solid
5 mg USD 270 In-stock
10 mg USD 405 In-stock
50 mg USD 1170 In-stock
100 mg USD 1890 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of TMC647055 Choline salt:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE TMC647055 Choline salt

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TMC647055 (Choline salt) is a potent nonnucleoside NS5B polymerase inhibitor of HCV replication. TMC647055 Choline salt has potent HCV combine activity with an IC50 value of 82 nM. TMC647055 Choline salt can be used for the research of Hepatitis C virus (HCV)[1][2].

IC50 & Target

EC90: 0.3 μM (Huh7-Luc cell)[1].
EC50: 82 nM (HCV)[2]

In Vitro

TMC647055 (Choline salt) has antiviral activity with an EC90 value of 0.3 μM in Huh7-Luc cells[1].
TMC647055 Choline salt has potent combine activity with an EC50 value of 82 nM in cellular HCV assays[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

TMC647055 Choline salt (compound 18a) (2 mg/kg, iv.; 10mg/kg, po.) shows an acceptable PK profile, characterized by high oral bioavailability and high systemic exposure after single oral dosing of 10 mg/kg, combined with a moderate plasma clearanceand low volume of distribution[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats[2]
Dosage: 2 mg/kg; 10mg/kg
Administration: 2 mg/kg, iv.; 10mg/kg, po.; singel
Result:
No. Cl (L/h/kg) Cmax(ng/mL) [Liver] (ng/mL) L/P F (%)
TMC647055 (compound 18a) 3.2 440 7800 46 >66
Clinical Trial
Molecular Weight

727.91

Formula

C37H53N5O8S

Appearance

Solid

Color

White to off-white

SMILES

OCC[N+](C)(C)C.COC1=CC2=C(C=C1)C3=C(C4CCCCC4)C5=CC=C(C6=O)C=C5N3CC(C(N(C)CCOCCN(S(N6)(=O)=O)C)=O)=C2.[OH-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (85.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3738 mL 6.8690 mL 13.7380 mL
5 mM 0.2748 mL 1.3738 mL 2.7476 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (2.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.82%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3738 mL 6.8690 mL 13.7380 mL 34.3449 mL
5 mM 0.2748 mL 1.3738 mL 2.7476 mL 6.8690 mL
10 mM 0.1374 mL 0.6869 mL 1.3738 mL 3.4345 mL
15 mM 0.0916 mL 0.4579 mL 0.9159 mL 2.2897 mL
20 mM 0.0687 mL 0.3434 mL 0.6869 mL 1.7172 mL
25 mM 0.0550 mL 0.2748 mL 0.5495 mL 1.3738 mL
30 mM 0.0458 mL 0.2290 mL 0.4579 mL 1.1448 mL
40 mM 0.0343 mL 0.1717 mL 0.3434 mL 0.8586 mL
50 mM 0.0275 mL 0.1374 mL 0.2748 mL 0.6869 mL
60 mM 0.0229 mL 0.1145 mL 0.2290 mL 0.5724 mL
80 mM 0.0172 mL 0.0859 mL 0.1717 mL 0.4293 mL
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TMC647055 Choline salt Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
TMC647055 Choline salt
Cat. No.:
HY-15591A
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