AcdK
AcdK is a non-natural amino acid and a precursor of allysine. AcdK allows site-specific incorporation into target proteins in E. coli via the amber suppression strategy. AcdK enables site-specific lysine dimethylation or monomethylation modification of target proteins. AcdK can synthesize site-specific lysine-methylated variants of histone H3 and p53, which is applicable for investigating the substrate specificity and catalytic function of epigenetic enzymes.
For research use only. We do not sell to patients.
- CAS No.: 2097535-26-5
- Formula: C14H17N5O4
- Molecular Weight:319.32
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
AcdK (0.5 mM) is efficiently and site-specifically incorporated into sfGFP at position D134 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable quantitative conversion to sfGFP with site-specific lysine dimethylation at position D134[1].
AcdK (2 mM) is efficiently and site-specifically incorporated into histone H3 at positions K4, K9, or K36 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable conversion to site-specific dimethylated histone H3 variants[1].
AcdK is efficiently and site-specifically incorporated into p53 at position K372 in E. coli BL21(DE3) cells, and subsequent biocompatible reactions enable conversion to p53 with site-specific lysine mono- or dimethylation at K372, which directly enhances Tip60-catalyzed acetylation of p53 at K120 by 2.5- to 3-fold[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2097535-26-5
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Molecular Weight 319.32
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Formula C14H17N5O4
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SMILES
OC([C@@H](N)CC/C=C/NC(OCC1=CC=C(C=C1)N=[N+]=[N-])=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
AcdK Related Classifications
HY-186091 Related Classifications
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