Adenosine 3'-phosphate 5'-phosphosulfate
Adenosine 3'-phosphate 5'-phosphosulfate, an adenine nucleotide derivative, is a selective P2Y1 antagonist with no effect on P2Y2, P2Y4, or P2Y6 receptors. Adenosine 3'-phosphate 5'-phosphosulfate can competitive inhibit ADP-induced platelet aggregation, as well as the ability of ADP to cause shape change and increases in Ca2+ in platelets, but had no effect on the inhibition of stimulated adenylate cyclase by ADP. Adenosine 3'-phosphate 5'-phosphosulfate is a co-substrate used for the sulfonation of glycans. Adenosine 3'-phosphate 5'-phosphosulfate can be used for Golgi-resident PAP-specific 3'-phosphatase-coupled sulfotransferase assays, which as donor substrate to transfer a sulfonate group.
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- CAS No.: 482-67-7
- Formula: C10H15N5O13P2S
- Molecular Weight:507.26
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
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- References
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Biological Activity
Description
IC50 & Target
[3]|
P2Y1 Receptor |
In Vitro
Adenosine 3'-phosphate 5'-phosphosulfate (10-100 μM) inhibits ADP-induced aggregation, shape change, and intracellular Ca2+ elevation in human platelets[3].
Adenosine 3'-phosphate 5'-phosphosulfate (100 μM) induces transient relaxation in the Carbamoylcholine chloride (HY-B1208)-preconstricted rat duodenum with no effect on the contraction of the vas deferens and bladder in male albino rats and BALB/c mice[3].
Adenosine 3'-phosphate 5'-phosphosulfate is degraded on the surface of the rat vas deferens with a T1/2 of 14.7 minutes, and the main degradation products is adenosine 3'-phosphate and 5'-phosphate[3].
Adenosine 3'-phosphate 5'-phosphosulfate is a high-energy sulfate donor for sulfating secretory macromolecules in the Golgi apparatus[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 482-67-7
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Molecular Weight 507.26
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Formula C10H15N5O13P2S
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SMILES
NC1=C2C(N([C@@H]3O[C@H](COP(OS(O)(=O)=O)(O)=O)[C@@H](OP(O)(O)=O)[C@H]3O)C=N2)=NC=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
References
[1]. Prather B, et, al. Golgi-resident PAP-specific 3'-phosphatase-coupled sulfotransferase assays. Anal Biochem. 2012 Apr 1;423(1):86-92. [Content Brief]
[2]. Ryan P. Modified indole alkaloids for therapeutic uses. WO2022133314.
[3]. Lüders F, et al. Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila. EMBO J. 2003 Jul 15;22(14):3635-44. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Adenosine 3'-phosphate 5'-phosphosulfate Related Classifications
HY-164090 Related Classifications
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