Art. -Nr.: HY-102082

Adenosine N1-oxide

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Adenosine N1-oxide is an oral active anti-inflammatory agent, and can be isolated from royal jelly. Adenosine N1-oxide promotes osteogenic and adipocyte differentiation.

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Reinheit: 99.50%
CAS. Nr.: 146-92-9
Formel: C₁₀H₁₃N₅O₅
Molecular Weight:283.24

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Adenosine N1-oxide Chemical Structure

CAS. Nr.: 146-92-9

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Adenosine N1-oxide is an oral active anti-inflammatory agent, and can be isolated from royal jelly. Adenosine N1-oxide promotes osteogenic and adipocyte differentiation^[1]^[2].

In Vitro

Adenosine N1-oxide )(1-40 μM, 24 h) inhibits TNF-α and IL-6 secretion by RAW264.7 cells stimulated with 2 µg/mL LPS (HY-D1056)^[2].
Adenosine N1-oxide )(20/100 μM, 30 mins) increases intracellular cAMP production in both peritoneal macrophages and RAW264.7 cells in a dose-dependent manner^[2].
Adenosine N1-oxide )(5-10 μM, 30 mins) increases the protein level of phosphorylation of Akt and GSK-3β in 2 µg/mL LPS (HY-D1056)-stimulated RAW264.7 cells^[2].
Adenosine N1-oxide )(2-10 μM, 6-7 days) promotes osteogenic and adipocyte differentiation in MC3T3-E1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

RAW264.7 cells
Concentration:

2, 5 and 10 μM
Incubation Time:

30 mins
Result:

Increased the protein level of phosphorylation of Akt and GSK-3β in 2 µg/mL LPS (HY-D1056)-stimulated RAW264.7 cells.

Cell Differentiation Assay^[2]

Cell Line:

MC3T3-E1 cells
Concentration:

2, 5 and 10 μM
Incubation Time:

6-7 days
Result:

Promoted osteogenic and adipocyte differentiation in MC3T3-E1 cells

In Vivo

Adenosine N1-oxide (oral administration, 135 mg/kg for three times) reduces the lethality caused by LPS (HY-D1056)-induced endotoxin shock in BALB/c mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

LPS-induced endotoxin shock in BALB/c mice^[1]
Dosage:

135 mg/kg for three times
Administration:

Oral administration
Result:

Reduced the lethality caused by LPS (HY-D1056)-induced endotoxin shock in BALB/c mice.

Chemical Information

CAS. Nr. 146-92-9
Appearance Solid
Molecular Weight 283.24
Formel C₁₀H₁₃N₅O₅
Color White to off-white
SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N(C=N2)C3=C2C(N)=[N+]([O-])C=N3)O1
Structure Classification
- Others
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (176.53 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5306 mL

17.6529 mL

35.3057 mL

5 mM

0.7061 mL

3.5306 mL

7.0612 mL

10 mM

0.3531 mL

1.7653 mL

3.5306 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kohno K, et al. Anti-inflammatory effects of adenosine N1-oxide. J Inflamm (Lond). 2015;12(1):2. Published 2015 Jan 20. [Content Brief]

[2]. Ohashi E, et al. Adenosine N1-Oxide Exerts Anti-inflammatory Effects through the PI3K/Akt/GSK-3β Signaling Pathway and Promotes Osteogenic and Adipocyte Differentiation. Biol Pharm Bull. 2019;42(6):968-976. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5306 mL	17.6529 mL	35.3057 mL	88.2644 mL
	5 mM	0.7061 mL	3.5306 mL	7.0611 mL	17.6529 mL
	10 mM	0.3531 mL	1.7653 mL	3.5306 mL	8.8264 mL
	15 mM	0.2354 mL	1.1769 mL	2.3537 mL	5.8843 mL
	20 mM	0.1765 mL	0.8826 mL	1.7653 mL	4.4132 mL
	25 mM	0.1412 mL	0.7061 mL	1.4122 mL	3.5306 mL
	30 mM	0.1177 mL	0.5884 mL	1.1769 mL	2.9421 mL
	40 mM	0.0883 mL	0.4413 mL	0.8826 mL	2.2066 mL
	50 mM	0.0706 mL	0.3531 mL	0.7061 mL	1.7653 mL
	60 mM	0.0588 mL	0.2942 mL	0.5884 mL	1.4711 mL
	80 mM	0.0441 mL	0.2207 mL	0.4413 mL	1.1033 mL
	100 mM	0.0353 mL	0.1765 mL	0.3531 mL	0.8826 mL

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Adenosine N1-oxide

Immunology/Inflammation Compound Library

Natural Product Library

Nucleotide Compound Library

Orally Active Compound Library

Osteogenesis Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Adenosine N1-oxide

Immunology/Inflammation Compound Library

Natural Product Library

Nucleotide Compound Library

Orally Active Compound Library

Osteogenesis Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Adenosine N1-oxide Related Classifications

HY-102082 Related Classifications