Albicanol
Albicanol is a sesquiterpenoid with potent antioxidant and antagonistic activities against heavy metal toxicity. Albicanol shows cytotoxicity. Albicanol suppress Profenofos (HY-B0832) induced genotoxicity in grass carp hepatocytes.
For research use only. We do not sell to patients.
- CAS No.: 54632-04-1
- Formula: C15H26O
- Molecular Weight:222.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Albicanol (compound 6) (0-30 µM; 48 h) shows cytotoxicity with IC50s of >30, 24.14, >30 µM for A549, MCF7, HepG2 cells, respectively[1].
Albicanol (0.00005 µg/mL; 24 h) significantly inhibits the genotoxicity of L8824 cells resulted from PFF exposure[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549, MCF7, HepG2 cells
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Concentration:0-30 µM
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Incubation Time:48 h
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Result:Showed cytotoxicity with IC50s of >30, 24.14, >30 µM for A549, MCF7, HepG2 cells, respectively.
Chemical Information
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CAS No. 54632-04-1
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Molecular Weight 222.37
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Formula C15H26O
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SMILES
CC(C)(CCC[C@@]1([C@H]2CO)C)[C@@H]1CCC2=C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Zhao DD, et al. Compounds from Dryopteris fragrans (L.) Schott with cytotoxic activity. Molecules. 2014 Mar 18;19(3):3345-55. [Content Brief]
[2]. Lihui X, et al. Albicanol modulates oxidative stress and the p53 axis to suppress profenofos induced genotoxicity in grass carp hepatocytes. Fish Shellfish Immunol. 2022 Mar;122:325-333. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Albicanol Related Classifications
HY-119635 Related Classifications
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