Compounds from Dryopteris fragrans (L.) Schott with cytotoxic activity

  • Molecules. 2014 Mar 18;19(3):3345-55. doi: 10.3390/molecules19033345.
Dan-Dan Zhao  1 ,  Qin-Shi Zhao  2 ,  Li Liu  3 ,  Zhong-Qin Chen  4 ,  Wei-Min Zeng  5 ,  Hong Lei  6 ,  Yan-Long Zhang  7
Affiliations
  • 1. Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. [email protected].
  • 2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China. [email protected].
  • 3. Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. [email protected].
  • 4. Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. [email protected].
  • 5. Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. [email protected].
  • 6. Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. [email protected].
  • 7. Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080, China. [email protected].
PMID: 24647035 DOI: 10.3390/molecules19033345
Abstract

One new coumarin, dryofracoumarin A (1), and eight known compounds 2-9 were isolated from Dryopteris fragrans (L.) Schott. Their structures were established on the basis of extensive spectroscopic data analyses and comparison with reported spectroscopic data. The new compound 1 was determined to be 8-hydroxyl-4-isopropyl-7-methyl-6-methyl-2H-benzopyran-2-one. Two dimers, trans- and cis-3-(3,4-dimethoxyphen-yl)-4-[(E)-3,4-dimethoxystyryl]cyclohex-1-ene (compounds 8 and 9), were isolated from the Dryopteris genus for the first time. The Other six were esculetin (2), isoscopoletin (3), methylphlorbutyrophenone (4), aspidinol (5), albicanol (6) and (E)-4-(3,4-dimethoxyphen-yl)but-3-en-1-ol (7). All compounds were evaluated for their cytotoxic effects by the MTT assay. Compounds 2, 3, 8 and 9 showed significantly cytotoxic effects against three cell lines (A549, MCF7 and HepG2), 1 and 5 against two cell lines (A549 and MCF7), and 6 against one cell line (MCF7). Their IC₅₀ values ranged between 2.73 ± 0.86 μM and 24.14 ± 3.12 μM. These active compounds might be promising lead compounds for the treatment of Cancer.

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