Ald-CH2-PEG3-azide
Ald-CH2-PEG3-azide is a cleavable 3 unit PEG ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Ald-CH2-PEG3-azide is a PEG-based PROTAC linker can be used in the synthesis of PROTACs. Ald-CH2-PEG3-azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- CAS No.: 1002342-83-7
- Formula: C8H15N3O4
- Molecular Weight:217.22
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
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Cleavable Linker |
PEGs |
In Vitro
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1002342-83-7
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Appearance Liquid
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Molecular Weight 217.22
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Formula C8H15N3O4
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Color Colorless to light yellow
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SMILES
O=CCOCCOCCOCCN=[N+]=[N-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (460.36 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.6036 mL | 23.0181 mL | 46.0363 mL | 115.0907 mL |
| 5 mM | 0.9207 mL | 4.6036 mL | 9.2073 mL | 23.0181 mL | |
| 10 mM | 0.4604 mL | 2.3018 mL | 4.6036 mL | 11.5091 mL | |
| 15 mM | 0.3069 mL | 1.5345 mL | 3.0691 mL | 7.6727 mL | |
| 20 mM | 0.2302 mL | 1.1509 mL | 2.3018 mL | 5.7545 mL | |
| 25 mM | 0.1841 mL | 0.9207 mL | 1.8415 mL | 4.6036 mL | |
| 30 mM | 0.1535 mL | 0.7673 mL | 1.5345 mL | 3.8364 mL | |
| 40 mM | 0.1151 mL | 0.5755 mL | 1.1509 mL | 2.8773 mL | |
| 50 mM | 0.0921 mL | 0.4604 mL | 0.9207 mL | 2.3018 mL | |
| 60 mM | 0.0767 mL | 0.3836 mL | 0.7673 mL | 1.9182 mL | |
| 80 mM | 0.0575 mL | 0.2877 mL | 0.5755 mL | 1.4386 mL | |
| 100 mM | 0.0460 mL | 0.2302 mL | 0.4604 mL | 1.1509 mL |
Ald-CH2-PEG3-azide Related Classifications
HY-130144 Related Classifications
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