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Cat. No.: HY-18052

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AM-8553

AM-8553 is a MDM2/p53 inhibitor (K_D: 0.4 nM for MDM2). AM-8553 has anti-tumor activity

For research use only. We do not sell to patients.

CAS No.: 1352064-70-0
Formula: C₂₅H₂₉Cl₂NO₄
Molecular Weight:478.41

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

AM-8553 Chemical Structure

CAS No.: 1352064-70-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Purity & Documentation
References
Calculators
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Classifications
Bioz

Biological Activity

Description

AM-8553 is a MDM2/p53 inhibitor (K_D: 0.4 nM for MDM2). AM-8553 has anti-tumor activity^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	>25 μM Compound: 29, AM-8553	Antiproliferative activity against p53 deficient human HCT116 cells assessed as reduction in BrdU incorporation after 16 hrs by Flow cytometry Antiproliferative activity against p53 deficient human HCT116 cells assessed as reduction in BrdU incorporation after 16 hrs by Flow cytometry	[PMID: 22524527]
HCT-116	IC₅₀	0.2 μM Compound: 29, AM-8553	Antiproliferative activity against human HCT116 cells expressing wild type p53 assessed as reduction in BrdU incorporation after 16 hrs by Flow cytometry Antiproliferative activity against human HCT116 cells expressing wild type p53 assessed as reduction in BrdU incorporation after 16 hrs by Flow cytometry	[PMID: 22524527]
SJSA-1	ED₅₀	78 mg/kg Compound: 29, AM-8553	Antitumor activity against human SJSA1 cells xenografted in po dosed athymic nude mouse assessed as tumor growth inhibition administered qd after 14 days Antitumor activity against human SJSA1 cells xenografted in po dosed athymic nude mouse assessed as tumor growth inhibition administered qd after 14 days	[PMID: 22524527]
SJSA-1	IC₅₀	0.068 μM Compound: 29, AM-8553	Antiproliferative activity against human SJSA1 cells after 3 days by EdU incorporation assay in presence of 10% human serum Antiproliferative activity against human SJSA1 cells after 3 days by EdU incorporation assay in presence of 10% human serum	[PMID: 22524527]
SJSA-1	IC₅₀	0.073 μM Compound: 1, AM-8553	Antiproliferative activity against human SJSA1 cells after 3 days by EdU incorporation-based HCS assay in presence of 10% human serum Antiproliferative activity against human SJSA1 cells after 3 days by EdU incorporation-based HCS assay in presence of 10% human serum	[PMID: 25147610]
SJSA-1	IC₅₀	0.76 μM Compound: 29, AM-8553	Activity at p53 in human SJSA1 cells assessed as induction of p21 mRNA expression after 7 hrs by qRT-PCR analysis in presence of 10% human serum Activity at p53 in human SJSA1 cells assessed as induction of p21 mRNA expression after 7 hrs by qRT-PCR analysis in presence of 10% human serum	[PMID: 22524527]
SJSA-1	IC₅₀	72 nM Compound: 1, AM-8553	Antiproliferative activity against human SJSA1 cells assessed as inhibition of EdU incorporation after 1 hr by Click-iT EdU HCS assay in presence of 10% human serum Antiproliferative activity against human SJSA1 cells assessed as inhibition of EdU incorporation after 1 hr by Click-iT EdU HCS assay in presence of 10% human serum	[PMID: 24456472]
SJSA-1	IC₅₀	760 nM Compound: 1, AM-8553	Antiproliferative activity against human SJSA1 cells assessed as induction of p21 gene level by qRT-PCR assay in presence of 10% human serum Antiproliferative activity against human SJSA1 cells assessed as induction of p21 gene level by qRT-PCR assay in presence of 10% human serum	[PMID: 24456472]

Chemical Information

CAS No. 1352064-70-0
Molecular Weight 478.41
Formula C₂₅H₂₉Cl₂NO₄
SMILES

O=C(O)C[C@]1(C)C(N([C@@H](CC)[C@@H](O)C)[C@H](C2=CC=C(Cl)C=C2)[C@@H](C3=CC=CC(Cl)=C3)C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bernard D, et al. AM-8553: a novel MDM2 inhibitor with a promising outlook for potential clinical development. J Med Chem. 2012 Jun 14;55(11):4934-5. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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