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Cat. No.: HY-148713

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Anti-CSCs agent-1

Anti-CSCs agent-1 is a potent anti-CSCs agent. Anti-CSCs agent-1 inhibits cell growth and cell migration. Anti-CSCs agent-1 induces Apoptosis. Anti-CSCs agent-1 inhibits the viability of CSCs. Anti-CSCs agent-1 enhances the production of ROS in CSCs. Anti-CSCs agent-1 shows antitumor activity.

For research use only. We do not sell to patients.

CAS No.: 2251753-58-7
Formula: C₄₄H₆₀FN₃O₄
Molecular Weight:713.96

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

Anti-CSCs agent-1 Chemical Structure

CAS No.: 2251753-58-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	0.7401 μM Compound: 48	Cytotoxicity activity against mouse 4T1 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against mouse 4T1 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
A-375	IC₅₀	0.8929 μM Compound: 48	Cytotoxicity activity against human A375 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human A375 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
A-375	IC₅₀	3.04 μM Compound: 48	Cytotoxicity against human A375 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay Cytotoxicity against human A375 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay	[PMID: 30433783]
A549	IC₅₀	11.56 μM Compound: 48	Cytotoxicity activity against human A549 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human A549 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
B16-F10	IC₅₀	0.6744 μM Compound: 48	Cytotoxicity activity against mouse B16F10 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against mouse B16F10 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
B16-F10	IC₅₀	1.24 μM Compound: 48	Cytotoxicity against mouse B16F10 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay Cytotoxicity against mouse B16F10 spheroid cells assessed as reduction in spheroid cell viability after 48 hrs by MTT assay	[PMID: 30433783]
Lewis lung carcinoma cell line	IC₅₀	0.9066 μM Compound: 48	Cytotoxicity activity against mouse LLC cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against mouse LLC cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
MDA-MB-231	IC₅₀	3.152 μM Compound: 48	Cytotoxicity activity against human MDA-MB-231 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human MDA-MB-231 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]
PANC-1	IC₅₀	1.107 μM Compound: 48	Cytotoxicity activity against human PANC1 cells assessed as cell growth inhibition after 48 hrs by MTT assay Cytotoxicity activity against human PANC1 cells assessed as cell growth inhibition after 48 hrs by MTT assay	[PMID: 30433783]

In Vitro

Anti-CSCs agent-1 (compound 48) (0, 0.25, 0.5 µM) inhibits the colony formation and cell migration in A375 and B16F10 cells in a dose-dependent manner^[1].
Anti-CSCs agent-1 (0, 1, 2, 3, 4 µM; 48 h) induces apoptosis with increases in the expression of cleaved PARP, cleaved caspase-3, P-53 and Bax in a dose-dependent manner^[1].
Anti-CSCs agent-1 (0, 0.25, 0.5, 1.0, 2.0 µM; 24 h) reverses epithelial-mesenchymal transition (EMT) by significantly upregulating the expression of E-cadherin in a dose-dependent manner^[1].
Anti-CSCs agent-1 (0-10 µM; 15 days) inhibits the viability of cancer stem cells (CSCs) with IC₅₀s of 3.04, 1.24 µM for spheriod A357, B16F10 cells, respectively^[1].
Anti-CSCs agent-1 (0-2 µM; 24 h) markedly enhances the production of ROS in CSCs in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

MDA-MB-231, 4T1, A375, B16F10, PANC-1, A549, LLC cells
Concentration:

0-50 µM
Incubation Time:

48 h
Result:

Showed antiproliferative activity with IC₅₀s of 3.152, 0.7401, 0.8929, 0.6744, 1.107, 11.56, 0.9066 µM for MDA-MB-231, 4T1, A375, B16F10, PANC-1, A549, LLC cells, respectively.

Apoptosis Analysis^[1]

Cell Line:

A375, B16F10 cells
Concentration:

0, 1, 2, 3 µM
Incubation Time:

48 h
Result:

Induced cell apoptosis in a dose-dependen manner.

Western Blot Analysis^[1]

Cell Line:

A375, B16F10 cells
Concentration:

0, 1, 2, 3, 4 µM
Incubation Time:

48 h
Result:

Increased the expression of cleaved PARP, cleaved casepase-3, P-53 and Bax in a dose-dependent manner.

In Vivo

Anti-CSCs agent-1 (5 mg/kg; i.p.; daily for 15 days) shows antitumor activity in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J mice (A375 cells)^[1]
Dosage:

5 mg/kg
Administration:

I.p.; daily for 15 days
Result:

Significantly inhibited tumor growth.

Chemical Information

CAS No. 2251753-58-7
Molecular Weight 713.96
Formula C₄₄H₆₀FN₃O₄
SMILES

O=C(C1(CC1)C(NC2=CC=C(C=C2)F)=O)NCCNC([C@]34[C@](CC(C)(CC4)C)([H])C5=CC[C@@]([C@@]6([C@@](C(C)(C(C(C6)=C)=O)C)([H])CC7)C)([H])[C@]7(C)[C@@]5(CC3)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liu X, et al. Synthesis and Discovery Novel Anti-Cancer Stem Cells Compounds Derived from the Natural Triterpenoic Acids. J Med Chem. 2018 Dec 13;61(23):10814-10833. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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