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Anti-inflammatory agent 36 is an anti-inflammatory agent. Anti-inflammatory agent 36 inhibits LPS-induced macrophage activation.

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Anti-inflammatory agent 36 Chemical Structure

Anti-inflammatory agent 36 Chemical Structure

CAS No. : 2293951-01-4

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Based on 1 publication(s) in Google Scholar

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Description

Anti-inflammatory agent 36 is an anti-inflammatory agent. Anti-inflammatory agent 36 inhibits LPS-induced macrophage activation[1].

In Vitro

Anti-inflammatory agent 36 (Compound 5a28) (2.5-20 μM) inhibits LPS-induced release of TNF-α and IL-6 in active RAW 264.7 mouse macrophages, with IC50s of 3.69 and 3.68 µM for TNF-α and IL-6[1].
Anti-inflammatory agent 36 (10 μM, 0.5 h) inhibits LPS-induced P-P38 and P-ERK in RAW 264.7 mouse macrophages[1].
Anti-inflammatory agent 36 (10 μM, 0.5 h) inhibits LPS-induced the transcription of TNF-α, IL-6, IL-1β, ICAM-1 and VCAM-1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: RAW 264.7 mouse macrophages
Concentration: 10 μM
Incubation Time: 0.5 h
Result: Markedly inhibited P-P38 and P-ERK, indicating the suppression of MAPK signaling.
In Vivo

Anti-inflammatory agent 36 (Compound 5a28) (10 mg/kg, i.p.) inhibits inflammation in acute lung injury mouse model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Acute lung injury mouse model[1]
Dosage: 10 mg/kg
Administration: i.p.
Result: Reduced wet/dry weight ratio of the mice lungs.
Reduced biomarkers of lymphocytes and macrophages.
Suppresses TNF-α, IL-6, IL-1β, 7 VACM-1 and ICAM-1 level.
Molecular Weight

453.48

Formula

C25H27NO7

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1N(C(/C=C/C2=CC=C(O)C(OC)=C2)=O)CCC/C1=C\C3=CC(OC)=C(OC)C(OC)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (55.13 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2052 mL 11.0258 mL 22.0517 mL
5 mM 0.4410 mL 2.2052 mL 4.4103 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.76 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2052 mL 11.0258 mL 22.0517 mL 55.1292 mL
5 mM 0.4410 mL 2.2052 mL 4.4103 mL 11.0258 mL
10 mM 0.2205 mL 1.1026 mL 2.2052 mL 5.5129 mL
15 mM 0.1470 mL 0.7351 mL 1.4701 mL 3.6753 mL
20 mM 0.1103 mL 0.5513 mL 1.1026 mL 2.7565 mL
25 mM 0.0882 mL 0.4410 mL 0.8821 mL 2.2052 mL
30 mM 0.0735 mL 0.3675 mL 0.7351 mL 1.8376 mL
40 mM 0.0551 mL 0.2756 mL 0.5513 mL 1.3782 mL
50 mM 0.0441 mL 0.2205 mL 0.4410 mL 1.1026 mL
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Anti-inflammatory agent 36 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Anti-inflammatory agent 36
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