Anti-inflammatory agent 36
Based on 1 Customer Validation
Anti-inflammatory agent 36 is an anti-inflammatory agent. Anti-inflammatory agent 36 inhibits LPS-induced macrophage activation.
For research use only. We do not sell to patients.
- Purity: 98.01%
- CAS No.: 2293951-01-4
- Formula: C25H27NO7
- Molecular Weight:453.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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IL-6 |
IL-1β |
TNF-α |
p38 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
3.68 μM
Compound: 5a28
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Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced IL6 production pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA
Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced IL6 production pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA
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[PMID: 30780088] |
| RAW264.7 | IC50 |
3.69 μM
Compound: 5a28
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Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced TNFalpha production pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA
Inhibition of MAPK/NFkappaB in ICR mouse RAW264.7 cells assessed as reduction in LPS-induced TNFalpha production pretreated for 0.5 hrs followed by LPS challenge and measured after 24 hrs by ELISA
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[PMID: 30780088] |
Anti-inflammatory agent 36 (Compound 5a28) (2.5-20 μM) inhibits LPS-induced release of TNF-α and IL-6 in active RAW 264.7 mouse macrophages, with IC50s of 3.69 and 3.68 µM for TNF-α and IL-6[1].
Anti-inflammatory agent 36 (10 μM, 0.5 h) inhibits LPS-induced P-P38 and P-ERK in RAW 264.7 mouse macrophages[1].
Anti-inflammatory agent 36 (10 μM, 0.5 h) inhibits LPS-induced the transcription of TNF-α, IL-6, IL-1β, ICAM-1 and VCAM-1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW 264.7 mouse macrophages
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Concentration:10 μM
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Incubation Time:0.5 h
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Result:Markedly inhibited P-P38 and P-ERK, indicating the suppression of MAPK signaling.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Acute lung injury mouse model[1]
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Dosage:10 mg/kg
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Administration:i.p.
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Result:Reduced wet/dry weight ratio of the mice lungs.
Reduced biomarkers of lymphocytes and macrophages.
Suppresses TNF-α, IL-6, IL-1β, 7 VACM-1 and ICAM-1 level.
Chemical Information
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CAS No. 2293951-01-4
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Appearance Solid
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Molecular Weight 453.48
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Formula C25H27NO7
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Color White to yellow
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SMILES
O=C1N(C(/C=C/C2=CC=C(O)C(OC)=C2)=O)CCC/C1=C\C3=CC(OC)=C(OC)C(OC)=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 25 mg/mL (55.13 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.76 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2052 mL | 11.0258 mL | 22.0517 mL | 55.1292 mL |
| 5 mM | 0.4410 mL | 2.2052 mL | 4.4103 mL | 11.0258 mL | |
| 10 mM | 0.2205 mL | 1.1026 mL | 2.2052 mL | 5.5129 mL | |
| 15 mM | 0.1470 mL | 0.7351 mL | 1.4701 mL | 3.6753 mL | |
| 20 mM | 0.1103 mL | 0.5513 mL | 1.1026 mL | 2.7565 mL | |
| 25 mM | 0.0882 mL | 0.4410 mL | 0.8821 mL | 2.2052 mL | |
| 30 mM | 0.0735 mL | 0.3675 mL | 0.7351 mL | 1.8376 mL | |
| 40 mM | 0.0551 mL | 0.2756 mL | 0.5513 mL | 1.3782 mL | |
| 50 mM | 0.0441 mL | 0.2205 mL | 0.4410 mL | 1.1026 mL |
Anti-inflammatory agent 36 Related Classifications
HY-148553 Related Classifications
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