Human CD235 Antibody (APC) (YA3400)
(Synonyms: Glycophorin-A/B; GYPA/B; MN sialoglycoprotein; SS-active sialoglycoprotein; PAS-2/3; Sialoglycoprotein alpha/delta; CD235a/b)Human CD235 Antibody (APC) (YA3400) is an APC-conjugated, mouse-derived, anti-Human CD235 IgG1 monoclonal antibody.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% Proclin300, 1% BSA.
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Conjugation:
APC
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 5μL per test for million cells or whole blood in 100 μL volume. |
Product Details
Human CD235 Antibody (APC) (YA3400) is an APC-conjugated, mouse-derived, anti-Human CD235 IgG1 monoclonal antibody.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 38 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 16 kDa
A synthesized peptide derived from Human CD235
Endogenous
affinity purified
APC
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% Proclin300, 1% BSA.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed:35835865). Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane; (Microbial infection) Appears to be a receptor for Hepatitis A virus (HAV); (Microbial infection) Receptor for P.falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Isoforms & Post-Translational Modification
P02724 has 3 isomers: P02724-1: 16430 Da (predicted); P02724-2: 13649 Da (predicted); P02724-3: 13000 Da (predicted).
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific) -
Subunit
Homodimer (PubMed:11313283, PubMed:1463744, PubMed:35835865). Component of the ankyrin-1 complex in the erythrocyte, composed of ANK1, RHCE, RHAG, SLC4A1, EPB42, GYPA, GYPB and AQP1 (PubMed:35835865). Interacts with SLC4A1; a GYPA monomer is bound at each end of the SLC4A1 dimer forming a heterotetramer (PubMed:35835865)
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SwissProt ID
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Synonyms
Glycophorin-A/B; GYPA/B; MN sialoglycoprotein; SS-active sialoglycoprotein; PAS-2/3; Sialoglycoprotein alpha/delta; CD235a/b
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