P2RY8 Antibody (YA4358)
(Synonyms: P2Y8)
Based on 1 Customer Validation
P2RY8 Antibody (YA4358) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to P2RY8.
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Host:
Mouse
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Isotype:
IgG2a
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Application:
IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:100-1:500 | 1:200-1:400 | 1:10000 |
Product Details
P2RY8 Antibody (YA4358) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to P2RY8.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 36 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 40.6 kDa
Purified recombinant fragment of human P2RY8 (AA: extra mix) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Flow cytometric analysis of 1X10^6 Hela cells labeling P2RY8 Antibody(HY-P84661 -
Flow cytometric analysis of 1X106 HeLa cells labeling P2RY8 Antibody (HY-P84661, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Immunocytochemistry analysis of HeLa cells labeling P2RY8 Antibody (HY-P84661) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with P2RY8 Antibody (HY-P84661) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit /Mouse IgG H&L（HY-P8002/HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunofluorescence analysis of Hela cells labeling P2RY8 antibody (HY-P84661) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
Background
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Function
P2RY8 is a G protein-coupled receptor for S-geranylgeranyl-glutathione (GGG), an endogenous metabolite present in lymphoid tissues. Couples the binding of GGG to the activation of GNA13 and downstream repression of AKT activation in lymphocytes defining their positioning and growth within lymphoid organs. In lymphoid follicles, confines B cells and follicular helper T cells in germinal centers (GCs) in response to GGG local gradients established by GGT5 (via GGG catabolism) and ABCC1 (via extracellular transport) with lower concentrations of GGG found in the follicular dendritic cell network region around which germinal centers are formed. In the bone marrow, also in response to GGG gradients established by GGT5 and ABCC1, it restricts chemotactic transmigration of B cells, T cells and NK cells from blood vessels to the bone marrow parenchyma. Contributes to GNA13-dependent pathway that suppresses GC B cell growth[1][2][3].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Barely detectable in normal blood leukocytes. Weaker expression was seen in heart, kidney and lung. Not detected in brain (PubMed:11004484, PubMed:15466006) . Expressed in B cells and follicular helper T cells in germinal centers (at protein level) (PubMed:30842656)
Induction:Down-regulated during granulocytic differentiation -
SwissProt ID
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Synonyms
P2Y8
Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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