Art. -Nr.: HY-W339331

Flubendiamide

(Synonyms: NNI-0001)

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Flubendiamide (NNI-0001) is an orally active phthalic diamide insecticide that acts by targeting insect ryanodine receptors (RyRs), causing insect muscle dysfunction, paralysis and death. Flubendiamide disrupts molting, metamorphosis and reproductive processes, induces oxidative stress by increasing the levels of ROS/RNS, MDA and 8OHdG and decreasing the levels of SOD, CAT and GST, activates the CncC/Maf apoptosis pathway, impairs calcium homeostasis, promotes adipogenesis, increases triglyceride accumulation, and upregulates the expression of regulatory factors for adipocyte differentiation and adipogenesis.

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Reinheit: 99.42%
CAS. Nr.: 272451-65-7
Formel: C₂₃H₂₂F₇IN₂O₄S
Molecular Weight:682.39

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Flubendiamide Chemical Structure

CAS. Nr.: 272451-65-7

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

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In Vitro

Flubendiamide (0-30 μM; 10-20 μM) binds to calf thymus DNA with moderate affinity via a groove-binding mechanism, with a binding constant of 49 μM, and alters the secondary structure of ct DNA^[1].
Flubendiamide (3 µM) induces persistent calcium transients in dissociated primary antennal neurons cultured in vitro from prepupae of the western honey bee Apis mellifera, with an average maximum amplitude of 0.50 ΔF/F^[2].
Flubendiamide (1-10 μM; 8 days) promotes triglyceride accumulation in 3T3-L1 adipocytes^[3].
Flubendiamide (1-10 μM; 8 days) upregulates adipogenic regulators C/EBPα and PPARγ at the concentration of 10 μM, and inhibits the AMPKα signaling pathway by reducing the phosphorylation levels of AMPKα and ACC; this effect is observed after treating 3T3-L1 adipocytes for 8 days, with a weaker effect detected at the concentration of 1 μM^[3].
Flubendiamide (10 μM; 8 days) enhances triglyceride accumulation in 3T3-L1 adipocytes, and this effect is reversed by co-treatment with 40 μM of the AMPKα activator AICAR^[3].
Flubendiamide (10 μM; 8 days) upregulates C/EBPα and inhibits the phosphorylation of AMPKα in 3T3-L1 adipocytes^[3].
Flubendiamide (10 μM; 8 days) promotes triglyceride accumulation in 3T3-L1 adipocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

3T3-L1 adipocytes
Concentration:

1 μM, 10 μM
Incubation Time:

8 days
Result:

Significantly increased protein expression of adipocyte differentiation regulators C/EBPα and PPARγ, and significantly decreased phosphorylation of AMPKα and its downstream target acetyl-CoA carboxylase (ACC) relative to control at 10 μM.
Resulted in a non-significant increase in C/EBPα and PPARγ expression, and a significant decrease in p-AMPKα/AMPKα and p-ACC/ACC ratios relative to control at 1 μM, though the magnitude of change was smaller than at 10 μM.

Western Blot Analysis^[3]

Cell Line:

3T3-L1 adipocytes
Concentration:

10 μM flubendiamide, 40 μM AICAR
Incubation Time:

8 days
Result:

Increased C/EBPα protein expression and decreased p-AMPKα/AMPKα ratios relative to control when used alone at 10 μM.
Abolished the flubendiamide-induced changes in C/EBPα and p-AMPKα expression when co-treated with 40 μM AICAR.
Abolished the flubendiamide-induced changes in C/EBPα and p-AMPKα expression when co-treated with 10 μM A769662.

In Vivo

Flubendiamide (20-80 μg/mL; oral administration; single exposure) exerts concentration- and time-dependent toxicity against Spodoptera litura^[1].
Flubendiamide (200 mg/kg; p.o.; daily; for 1, 7 or 14 days) bioaccumulates in the liver and adipose tissue of rats. After daily oral administration at a dose of 200 mg/kg for 1-14 days, its concentrations reach 19-27 mg/kg in the liver and 47-68 mg/kg in adipose tissue^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

3rd generation, 4th instar larvae, 120 h old, uniform size^[1]
Dosage:

20 μg/mL; 40 μg/mL; 60 μg/mL; 80 μg/mL
Administration:

oral; single exposure; observation over 12-72 hours
Result:

Induced concentration- and time-dependent toxicity in Spodoptera litura, including 45.33% larval mortality at 80 μg/mL (24 hours), oxidative stress, genotoxic damage, gut tissue injury, disrupted life cycle and reproduction, and altered expression of stress, apoptotic, and ecdysone receptor genes.

Animal Model:

Fischer F344/DuCrj (female)^[3]
Dosage:

200 mg/kg
Administration:

p.o.; daily; 1, 7, or 14 days
Result:

Reached peak plasma levels of ~20 µM after oral daily dosing for 1, 7, or 14 days.
Accumulated to liver levels of 19-27 mg/kg after oral daily dosing for 1, 7, or 14 days.
Accumulated to fat tissue levels of 47-68 mg/kg after oral daily dosing for 1, 7, or 14 days.

Chemical Information

CAS. Nr. 272451-65-7
Appearance Solid
Molecular Weight 682.39
Formel C₂₃H₂₂F₇IN₂O₄S
Color White to off-white
SMILES

O=C(C1=C(C(NC(C)(CS(=O)(C)=O)C)=O)C(I)=CC=C1)NC2=CC=C(C(C(F)(F)F)(C(F)(F)F)F)C=C2C
Synonyms

NNI-0001
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (183.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4654 mL

7.3272 mL

14.6544 mL

5 mM

0.2931 mL

1.4654 mL

2.9309 mL

10 mM

0.1465 mL

0.7327 mL

1.4654 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (399 KB)
- English - EN (399 KB)
- Français - FR (399 KB)
- Deutsch - DE (399 KB)
- Norwegian - NO (399 KB)
- Español - ES (399 KB)
- Swedish - SV (399 KB)
- Italian - IT (399 KB)
- Korean - KR (399 KB)
- Portuguese - PT (399 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Jameel M, et al. Flubendiamide induced genetic and cellular damages directly influence the life cycle of the oriental leaf worm, Spodoptera litura. Pestic Biochem Physiol. 2023;193:105448. [Content Brief]

[2]. Kadala A, et al. Flubendiamide, the first phthalic acid diamide insecticide, impairs neuronal calcium signalling in the honey bee's antennae. J Insect Physiol. 2020;125:104086. [Content Brief]

[3]. Sun Q, et al. Flubendiamide Enhances Adipogenesis and Inhibits AMPKα in 3T3-L1 Adipocytes. Molecules. 2018 Nov 12;23(11):2950.
[Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4654 mL	7.3272 mL	14.6544 mL	36.6359 mL
	5 mM	0.2931 mL	1.4654 mL	2.9309 mL	7.3272 mL
	10 mM	0.1465 mL	0.7327 mL	1.4654 mL	3.6636 mL
	15 mM	0.0977 mL	0.4885 mL	0.9770 mL	2.4424 mL
	20 mM	0.0733 mL	0.3664 mL	0.7327 mL	1.8318 mL
	25 mM	0.0586 mL	0.2931 mL	0.5862 mL	1.4654 mL
	30 mM	0.0488 mL	0.2442 mL	0.4885 mL	1.2212 mL
	40 mM	0.0366 mL	0.1832 mL	0.3664 mL	0.9159 mL
	50 mM	0.0293 mL	0.1465 mL	0.2931 mL	0.7327 mL
	60 mM	0.0244 mL	0.1221 mL	0.2442 mL	0.6106 mL
	80 mM	0.0183 mL	0.0916 mL	0.1832 mL	0.4579 mL
	100 mM	0.0147 mL	0.0733 mL	0.1465 mL	0.3664 mL

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Flubendiamide

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

NF-κB Signaling Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Orally Active Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Mitochondrial Toxicity Compound Library

Cell Death Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Pesticide Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Alle Calcium Channel Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Flubendiamide

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

NF-κB Signaling Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Orally Active Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Mitochondrial Toxicity Compound Library

Cell Death Library

Ion Channel Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Pesticide Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Alle Calcium Channel Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Flubendiamide Related Classifications

HY-W339331 Related Classifications