Art. -Nr.: HY-17556

Folinic acid

Name: Folinic acid
Brand: MedChemExpress
SKU: HY-17556
Price: 66.00 USD
Availability: InStock
Rating: 5 (18 reviews)

(Synonyms: leucovorin)

18 Cited Publications

Kundenbewertung

Based on 18 publication(s) in Google Scholar

Folinic acid (Leucovorin) is a biological folic acid and is generally administered along with Methotrexate (HY-14519) as a rescue agent to decrease MTX-induced toxicity.

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Reinheit : 99.98%
CAS. Nr.: 58-05-9
Formel: C₂₀H₂₃N₇O₇
Molecular Weight:473.44

Speicherung:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen)

Folinic acid Chemical Structure

CAS. Nr.: 58-05-9

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Folinic acid (Leucovorin) is a biological folic acid and is generally administered along with Methotrexate (HY-14519) as a rescue agent to decrease MTX-induced toxicity^[1].

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

Methotrexate (MTX) alone induces a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There is a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreases in all concentrations of MTX tested. The addition of Folinic acid at 50 μg/ mL significantly reduces % MNBN (40-68%) and % Abs (36-77%). Inhibition is also seen at 5 μg/ mL Folinic acid (12 to 54% for MNBN and 20 to 61% for Abs) ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Folinic acid (7.0 mg/kg; intraperitoneal injection; every second day; for 3 weeks; Balb/c young growing male mice) treatment following Methotrexate (MTX) administration appears to reverse this growth inhibition (Chronic administration of MTX induces suppression of skeletal growth in mice)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

24 Balb/c young growing male mice aged 3 weeks (11.88 ± 0.25 g)^[2]
Dosage:

7.0 mg/kg
Administration:

Intraperitoneal injection; every second day; for 3 weeks
Result:

Following MTX administration appears to reverse this growth inhibition.

Chemical Information

CAS. Nr. 58-05-9
Appearance Solid
Molecular Weight 473.44
Formel C₂₀H₂₃N₇O₇
Color Off-white to light yellow
SMILES

O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(NCC2N(C=O)C3=C(N=C(N)NC3=O)NC2)C=C1)=O
Synonyms

leucovorin
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen)

Publications (18)

All In Vivo Efficacy StudyFlow CytometryWBBio/Physico-chemical Assay

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Journal Impact Factor

Signal Transduct Target Ther

Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]

2025 Dec 15;10(1):406. PMID: 41392286
Cancer Cell

Chemotherapy triggers immune evasion by fostering LEPR+ Kupffer cell differentiation in liver metastases. [Abstract]

2026 Mar 9;44(3):658-675.e12. PMID: 41687606
JAMA Oncol

Chemoradiotherapy Plus Induction or Consolidation Chemotherapy as Total Neoadjuvant Therapy for Patients With Locally Advanced Rectal Cancer: Long-term Results of the CAO/ARO/AIO-12 Randomized Clinical Trial. [Abstract]

2022 Jan 1;8(1):e215445. PMID: 34792531
Nat Commun

Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. [Abstract]

2020 Apr 14;11(1):1792. PMID: 32286289
EBioMedicine

Desulfovibrio desulfuricans and its derived metabolites confer resistance to FOLFOX through METTL3. [Abstract]

2024 Apr:102:105041. PMID: 38484555
NPJ Precis Oncol

Single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer. [Abstract]

2023 Dec 8;7(1):128. PMID: 38066116
Mol Cancer Ther

Mosperafenib, a novel paradox breaker BRAF inhibitor with potent preclinical activity in BRAF mutated colorectal cancer. [Abstract]

2025 Dec 4. PMID: 41340484
J Mol Med (Berl)

Combination of PARP inhibitor and temozolomide to suppress chordoma progression. [Abstract]

2019 Aug;97(8):1183-1193. PMID: 31201471
Appl Microbiol Biotechnol

Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model. [Abstract]

2024 Dec;108(1):1-15. PMID: 38175236
Mol Oncol

Patient therapy outcome modeling in cancer organoids is improved by cancer-associated fibroblasts and organoid assembly convolution. [Abstract]

2026 Jun 5. PMID: 42246237
Mol Oncol

Optimized low-dose combinatorial drug treatment boosts selectivity and efficacy of colorectal carcinoma treatment. [Abstract]

2020 Nov;14(11):2894-2919. PMID: 33021054

Folinic acid purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]

FOLFOX was administered as 6 mg/kg Oxaliplatin two hours before administration of 90 mg/kg Leucovorin and 50 mg/kg 5-Fluorouracil at day 1 and a repeat of 5‐Fluorouracil dosing on day 2. Weight loss of ODC-, CTRL-, and FOLFOX-treated mice over time of mice with DLD1 tumors.

Folinic acid purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]

The corresponding panels show the activity of ODC, the corresponding monotherapy, and FOLFOX (0.5 µM Leucovorin, 5-Fluorouracil, Oxaliplatin), measured by comparing ATP levels in CRC cells (colored bars) and healthy CCD841 colonic epithelial cells (black bars) with CTRL (<0.15% DMSO), thus obtaining the treatment window (TW).
Hum Mol Genet

2026 Jul 28;35(16):ddag071. PMID: 42520321
Int J Immunopathol Pharmacol

Chemotherapy elevates cell surface PD-L1 and MHC-I expression in apoptotic gastric cancer cells. [Abstract]

2025 Jan-Dec:39:3946320251338662. PMID: 40380807

Folinic acid purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]

Effects of FOLFOX on surface PD-L1 expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and PD-L1 antibody. Representative histogram showing surface PD-L1 expression. Red histogram: total cells. Purple histogram: annexin V-positive cells.

Folinic acid purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]

Effects of FOLFOX on surface MHC-I expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and MHC-I antibody. Representative dot plots showing the expression of annexin V and surface MHC-I on AGS cells.
Virology

Structure-based virtual screening of ROCK1 inhibitors for the discovery of Enterovirus-A71 antivirals. [Abstract]

2023 Aug:585:205-214. PMID: 37384967

Folinic acid purchased from MedChemExpress. Usage Cited in: Virology. 2023 Aug:585:205-214. [Abstract]

Leucovorin (10 μM; 48 h). VP1 protein levels were detected using Western blot.
Oncol Lett

Leucovorin enhances curcumin-induced inhibition of cell viability and ferroptosis in colorectal cancer cells. [Abstract]

2026 Mar 4;31(5):155. PMID: 41852897
bioRxiv

2025 Sep 17.
Patent

US20230101335A1.
bioRxiv

2023 Feb 28.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 175 mg/mL (369.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1122 mL

10.5610 mL

21.1220 mL

5 mM

0.4224 mL

2.1122 mL

4.2244 mL

10 mM

0.2112 mL

1.0561 mL

2.1122 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.39 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Keshava, C., et al., Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutat Res, 1998. 397(2): p. 221-8. [Content Brief]

[2]. Iqbal MP, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatr. 2003 Dec;92(12):1438-44. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1122 mL	10.5610 mL	21.1220 mL	52.8050 mL
	5 mM	0.4224 mL	2.1122 mL	4.2244 mL	10.5610 mL
	10 mM	0.2112 mL	1.0561 mL	2.1122 mL	5.2805 mL
	15 mM	0.1408 mL	0.7041 mL	1.4081 mL	3.5203 mL
	20 mM	0.1056 mL	0.5281 mL	1.0561 mL	2.6403 mL
	25 mM	0.0845 mL	0.4224 mL	0.8449 mL	2.1122 mL
	30 mM	0.0704 mL	0.3520 mL	0.7041 mL	1.7602 mL
	40 mM	0.0528 mL	0.2640 mL	0.5281 mL	1.3201 mL
	50 mM	0.0422 mL	0.2112 mL	0.4224 mL	1.0561 mL
	60 mM	0.0352 mL	0.1760 mL	0.3520 mL	0.8801 mL
	80 mM	0.0264 mL	0.1320 mL	0.2640 mL	0.6601 mL
	100 mM	0.0211 mL	0.1056 mL	0.2112 mL	0.5281 mL

Bioz

Keywords

Folinic acid

18 Cited Publications

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Folinic acid

Publications Citing Use of MedChemExpress (MCE) Folinic acid

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen