Folinic acid disodium
Based on 13 publication(s) in Google Scholar
Folinic acid (Leucovorin) disodium is a biological folic acid and is a forms of vitamin B9. Folinic acid disodium is generally administered along with Methotrexate (HY-14519) as a rescue agent to decrease MTX-induced toxicity. Folinic acid disodium and Sfluorouracil adjuvant chemotherapy shows effective in colon carcinoma.
For research use only. We do not sell to patients.
- Purity : 99.76%
- CAS No.: 163254-40-8
- Formula: C20H21N7Na2O7
- Molecular Weight:517.40
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-10820
Pemetrexed -
HY-13664
Folinic acid calciumBiological Folic Acid -
HY-17556
Folinic acidBiological Folic Acid -
HY-10446
PralatrexateDihydrofolate Reductasean Inhibitor -
HY-10820R
Pemetrexed (Standard) -
HY-13667
Levoleucovorin CalciumAntineoplastic Agent -
HY-17383
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HY-13781
Pemetrexed disodium hemipenta hydrateAutophagy Inducer -
HY-14530
PelitrexolGARFT Inhibitor -
HY-B0080
Folinic acid calcium salt pentahydrateEndogenous Metabolite -
HY-17557
Calcium N5-methyltetrahydrofolateFolic Acid Supplement -
HY-10818
LY309887
Publications Citing Use of MedChemExpress (MCE) Folinic acid disodium
More- Cancer Cell. 2026 Mar 9;44(3):658-675.e12. [Abstract]
- JAMA Oncol. 2022 Jan 1;8(1):e215445. [Abstract]
- NPJ Precis Oncol. 2023 Dec 8;7(1):128. [Abstract]
- Appl Microbiol Biotechnol. 2024 Dec;108(1):1-15. [Abstract]
- Mol Oncol. 2026 Jun 5. [Abstract]
- Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]
- Hum Mol Genet. 2026 Jul 28;35(16):ddag071.
- Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]
- Virology. 2023 Aug:585:205-214. [Abstract]
- Oncol Lett. 2026 Mar 4;31(5):155. [Abstract]
- bioRxiv. 2025 Sep 17.
- Patent. US20230101335A1.
- bioRxiv. 2023 Feb 28.
Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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WB
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In Vivo Efficacy Study
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
Folinic acid disodium (5, 50 μg/ml; 40 h) significantly reduces % micronucleated binucleated cells (MNBN) (40%-68%) and % aberrant cells (Abs) (36%-77%) in V79 cells that were treated with MTX at five different concentrations 5–100 mg/ml for 6 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:24 Balb/c young growing male mice aged 3 weeks (11.88 ± 0.25 g)[2]
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Dosage:7.0 mg/kg
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Administration:Intraperitoneal injection; every second day; for 3 weeks
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Result:Following MTX administration appears to reverse this growth inhibition.
Chemical Information
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CAS No. 163254-40-8
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Appearance Solid
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Molecular Weight 517.40
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Formula C20H21N7Na2O7
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Color Off-white to light yellow
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SMILES
O=C(O[Na])CC[C@@H](C(O[Na])=O)NC(C1=CC=C(NCC2N(C=O)C3=C(NC(N)=NC3=O)NC2)C=C1)=O
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Synonyms
Leucovorin disodium
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (13)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Chemotherapy triggers immune evasion by fostering LEPR+ Kupffer cell differentiation in liver metastases. [Abstract]2026 Mar 9;44(3):658-675.e12. PMID: 41687606 -
JAMA Oncol
Chemoradiotherapy Plus Induction or Consolidation Chemotherapy as Total Neoadjuvant Therapy for Patients With Locally Advanced Rectal Cancer: Long-term Results of the CAO/ARO/AIO-12 Randomized Clinical Trial. [Abstract]2022 Jan 1;8(1):e215445. PMID: 34792531 -
NPJ Precis Oncol
Single-organoid analysis reveals clinically relevant treatment-resistant and invasive subclones in pancreatic cancer. [Abstract]2023 Dec 8;7(1):128. PMID: 38066116 -
Appl Microbiol Biotechnol
Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model. [Abstract]2024 Dec;108(1):1-15. PMID: 38175236 -
Mol Oncol
Patient therapy outcome modeling in cancer organoids is improved by cancer-associated fibroblasts and organoid assembly convolution. [Abstract]2026 Jun 5. PMID: 42246237 -
Mol Oncol
Optimized low-dose combinatorial drug treatment boosts selectivity and efficacy of colorectal carcinoma treatment. [Abstract]2020 Nov;14(11):2894-2919. PMID: 33021054
Folinic acid disodium purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]
FOLFOX was administered as 6 mg/kg Oxaliplatin two hours before administration of 90 mg/kg Leucovorin and 50 mg/kg 5-Fluorouracil at day 1 and a repeat of 5‐Fluorouracil dosing on day 2. Weight loss of ODC-, CTRL-, and FOLFOX-treated mice over time of mice with DLD1 tumors.
Folinic acid disodium purchased from MedChemExpress. Usage Cited in: Mol Oncol. 2020 Nov;14(11):2894-2919. [Abstract]
The corresponding panels show the activity of ODC, the corresponding monotherapy, and FOLFOX (0.5 µM Leucovorin, 5-Fluorouracil, Oxaliplatin), measured by comparing ATP levels in CRC cells (colored bars) and healthy CCD841 colonic epithelial cells (black bars) with CTRL (<0.15% DMSO), thus obtaining the treatment window (TW).
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Int J Immunopathol Pharmacol
Chemotherapy elevates cell surface PD-L1 and MHC-I expression in apoptotic gastric cancer cells. [Abstract]2025 Jan-Dec:39:3946320251338662. PMID: 40380807
Folinic acid disodium purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]
Effects of FOLFOX on surface PD-L1 expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and PD-L1 antibody. Representative histogram showing surface PD-L1 expression. Red histogram: total cells. Purple histogram: annexin V-positive cells.
Folinic acid disodium purchased from MedChemExpress. Usage Cited in: Int J Immunopathol Pharmacol. 2025 Jan-Dec:39:3946320251338662. [Abstract]
Effects of FOLFOX on surface MHC-I expression on AGS cells. Cells were treated with FOLFOX (Leucovorin: 14 μM; 5-Fu: 200 μM; Oxaliplatin: 6 μM) for 48 h. Then, cells were co-stained with annexin V and MHC-I antibody. Representative dot plots showing the expression of annexin V and surface MHC-I on AGS cells.
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Virology
Structure-based virtual screening of ROCK1 inhibitors for the discovery of Enterovirus-A71 antivirals. [Abstract]2023 Aug:585:205-214. PMID: 37384967
Folinic acid disodium purchased from MedChemExpress. Usage Cited in: Virology. 2023 Aug:585:205-214. [Abstract]
Leucovorin (10 μM; 48 h). VP1 protein levels were detected using Western blot.
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Oncol Lett
Leucovorin enhances curcumin-induced inhibition of cell viability and ferroptosis in colorectal cancer cells. [Abstract]2026 Mar 4;31(5):155. PMID: 41852897 -
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Solvent & Solubility
In Vitro:
H2O : 2 mg/mL (3.87 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Keshava, C., et al., Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutat Res, 1998. 397(2): p. 221-8. [Content Brief]
[2]. Iqbal MP, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatr. 2003 Dec;92(12):1438-44. [Content Brief]
[3]. Francini G, et al. Folinic acid and 5-fluorouracil as adjuvant chemotherapy in colon cancer. Gastroenterology. 1994 Apr;106(4):899-906. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.9327 mL | 9.6637 mL | 19.3274 mL | 48.3185 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Folinic acid disodium Related Classifications
HY-17556A Related Classifications
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