Phos-FLAG
Phos-FLAG is a phosphine probe containing the Flag peptide. Phos-FLAG undergoes Staudinger ligation with azido groups metabolically incorporated into cell surface glycans and organ glycoproteins, enabling quantitative analysis of the presence of cell surface azides. Phos-FLAG detects azide-labeled glycans in isolated cells and organ lysates.
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- CAS. Nr.: 403481-00-5
- Formel: C62H75N10O23P
- Molecular Weight:1359.29
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
In Vivo
Phos-FLAG (16 μM; i.p.; single dose; 24 h after final Ac4ManNAz injection) successfully mediates Staudinger ligation with metabolically introduced azide groups on mouse splenocytes in vivo, generating detectable cell-surface Flag epitopes with no observed toxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Es1^e/Es1^e mice (plasma esterase-deficient, male and female); B6D2F1 mice (wild-type, male and female)[1]
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Dosage:250 μM (ex vivo incubation for 1 h); 500 μM (ex vivo incubation for 6-12 h)
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Administration:ex vivo incubation; 1 h (250 μM); 6-12 h (500 μM); room temperature
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Result:Exhibited a dose-dependent increase in mean fluorescence intensity (MFI) in splenocytes from Ac4ManNAz-treated mice, with MFI values significantly higher than vehicle-treated controls.
Detected discrete labelled glycoprotein bands in heart, kidney, and liver lysates from Ac4ManNAz-treated mice, but not in brain or thymus homogenates.
Significantly reduced MFI after Phos-Flag labelling in sialidase-treated splenocytes from Ac4ManNAz-treated mice, confirming azide label presence on sialic acids.
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Animal Model:C57BL/6 mice[1]
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Dosage:16 μM
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Administration:i.p.; single dose; 24 hours after final Ac4ManNAz injection
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Result:Showed a significant increase in MFI in splenocytes from mice treated with both Ac4ManNAz and Phos-Flag relative to untreated mice or mice treated with only one agent.
Produced a larger MFI increase in ex vivo Phos-Flag treatment of splenocytes from mice given only Ac4ManNAz compared to ex vivo treatment of splenocytes from mice given both Ac4ManNAz and in vivo Phos-Flag, indicating significant in vivo Staudinger ligation of cell-surface azides.
Observed no adverse physiological effects in mice after administration.
Chemical Information
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CAS. Nr. 403481-00-5
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Molecular Weight 1359.29
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Formel C62H75N10O23P
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SMILES
O=C(OC)C(C=C1)=C(P(C2=CC=CC=C2)C3=CC=CC=C3)C=C1C(N[C@@H](CC(O)=O)C(N[C@@H](CC4=CC=C(C=C4)O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Phos-FLAG Related Classifications
HY-P11710 Related Classifications
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