PYSNO
PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λem=515-565 nm, λex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases.
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- CAS. Nr.: 2445485-84-5
- Formel: C28H31N5O3S
- Molecular Weight:517.64
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
In cell-free fluorescence spectroscopy assays, PYSNO (5 μM; 10 min, 60 s, 15 s) rapidly detects NO with a 23-fold fluorescence enhancement, a linear response range of 1 to 30 μM, and a limit of detection of 242 nM[1].
PYSNO (5 μM; 30 min) selectively accumulates in lysosomes of SH-SY5Y human neuroblastoma cells, as confirmed by a Pearson’s colocalization coefficient of 0.87 with Lyso-Tracker Red (HY-D1300) after NO-induced fluorescence activation[1].
PYSNO (5 μM; 30 min) detects exogenous NO in SH-SY5Y human neuroblastoma cells with dose-dependent fluorescence activation, showing a 10.5-fold enhancement at 200 μM NO[1].
PYSNO (5 μM; 30 min) detects endogenously produced NO in RAW264.7 mouse macrophages, showing a 2.8-fold fluorescence enhancement after NO induction, with signal reduction by NOS inhibition or NO scavenging[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice (male, 10 weeks old; myocardial fibrosis induced by subcutaneous isoproterenol injection)[1]
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Dosage:50 μM (200 μL, 0.2% DMSO in PBS)
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Administration:i.v. (tail vein); single dose
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Result:Showed highly enhanced fluorescence in the heart region of myocardial fibrosis model mice relative to normal mice in in vivo imaging.
Confirmed significantly higher fluorescence intensity in myocardial fibrosis model mice relative to normal mice in ex vivo imaging of harvested hearts.
Chemical Information
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CAS. Nr. 2445485-84-5
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Molecular Weight 517.64
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Formel C28H31N5O3S
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SMILES
NC1=C(C=C(C=C1)C2=CC=C(S2)N3N=C(C=CC3=O)C4=CC=C(C=C4)OCCCCN5CCOCC5)N
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
[1]. Zhou T, et al. A Smart Fluorescent Probe for NO Detection and Application in Myocardial Fibrosis Imaging. Anal Chem. 2020;92(7):5064-5072. [Content Brief]
[2]. Dong X, et al. Theoretical insights into the linker effects on the turn-on fluorescence behaviors in pyridazinone-containing NO probes. Spectrochim Acta A Mol Biomol Spectrosc. 2024;321:124761. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
PYSNO Related Classifications
HY-D3250 Related Classifications
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