Art. -Nr.: HY-120075

TJ191

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TJ191 is a potent and specific anti-cancer agent that targets low TβRIII-expressing malignant T-cell leukemia/lymphoma cells. TJ191 has no affects on the proliferation of other cancer cells or normal fibroblasts or immune cells. TJ191 can be used for cancer research.

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Reinheit: 99.83%
CAS. Nr.: 1522415-97-9
Formel: C₁₃H₂₁NO₂S
Molecular Weight:255.38

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

TJ191 Chemical Structure

CAS. Nr.: 1522415-97-9

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
CAKI-1	IC₅₀	0.54 μM Compound: 3j	Cytotoxicity against human Caki1 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human Caki1 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
CCRF-CEM	IC₅₀	>=34 μM Compound: 3j	Antiproliferative activity against human CEM cells passaged every 4 to 5 days in presence of compound up to 10 uM Antiproliferative activity against human CEM cells passaged every 4 to 5 days in presence of compound up to 10 uM	[PMID: 28365318]
CCRF-CEM	IC₅₀	0.18 μM Compound: 3j	Cytotoxicity against human CEM cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human CEM cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
CCRF-CEM	IC₅₀	0.9 μM Compound: 8	Antiproliferative activity against human CEM cells assessed as reduction in cell viability Antiproliferative activity against human CEM cells assessed as reduction in cell viability	[PMID: 28987607]
HeLa	IC₅₀	81 μM Compound: 3j	Cytotoxicity against human HeLa cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human HeLa cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
Huh-7	IC₅₀	0.34 μM Compound: 3j	Cytotoxicity against human HuH7 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human HuH7 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
MOLT-4	IC₅₀	0.057 μM Compound: 3j	Cytotoxicity against human MOLT4 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human MOLT4 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
PBMC	IC₅₀	>50 μM Compound: 3j	Cytotoxicity against human PBMC cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human PBMC cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
PC-3	IC₅₀	>=34 μM Compound: 3j	Antiproliferative activity against human PC3 cells passaged every 4 to 5 days in presence of compound up to 10 uM Antiproliferative activity against human PC3 cells passaged every 4 to 5 days in presence of compound up to 10 uM	[PMID: 28365318]
PC-3	IC₅₀	0.62 μM Compound: 3j	Cytotoxicity against human PC3 cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human PC3 cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]
Raji	IC₅₀	105 μM Compound: 3j	Cytotoxicity against human Raji cells after 72 to 96 hrs by coulter counter method Cytotoxicity against human Raji cells after 72 to 96 hrs by coulter counter method	[PMID: 28365318]

In Vitro

TJ191(0-100 μM; 24 hours) exhibits pronounced anti-proliferative activity in malignant cell lines, the IC₅₀ values are 0.13 μM, 0.13±0.08 μM, 0.26±0.19 μM, 0.22±0.11 μM, 1.5±0.02 μM, 0.32±0.086 μM, 3.1±0.5 μM, 0.26±0.16 μM in CEM, JURKAT, MOLT-3, MOLT-4, SUP-T1, MT-2, C8166 and HSB-2 cells, respectively. But has no effects in HUT-78 (IC₅₀=17±10 μM) and MT-4 (IC₅₀=47 ± 5 μM) cells^[1].
TJ191 (0.1-3 μM; 8 or 24 hours) induces CEM cell apoptosis in a concentration- and time-dependent manner. Even at 0.3 μM, TJ191 induces the maximum apoptotic rate of 80% after 24 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Malignant T-cell leukemia/lymphoma cells
Concentration:

0.01 µM, 0.1 µM, 1 µM, 10 µM, 100 µM
Incubation Time:

24 hours
Result:

Exhibited inhibitory effects in drug-sensitive cells with IC₅₀ ranging 0.13 µM to 3.1 µM.

Apoptosis Analysis^[1]

Cell Line:

CEM cell line
Concentration:

0.1-3 µM
Incubation Time:

8 or 24 hours
Result:

Led to cell apoptosis.

Chemical Information

CAS. Nr. 1522415-97-9
Appearance Solid
Molecular Weight 255.38
Formel C₁₃H₂₁NO₂S
Color Light yellow to khaki
SMILES

O=C(C1=C(N)SC(CCCCCCC)=C1)OC
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (391.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9157 mL

19.5787 mL

39.1573 mL

5 mM

0.7831 mL

3.9157 mL

7.8315 mL

10 mM

0.3916 mL

1.9579 mL

3.9157 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (9.79 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Ahmed El-Gazzar, et al. 2-Amino-3-methylcarboxy-5-heptyl-thiophene (TJ191) is a selective anti-cancer small molecule that targets low TβRIII-expressing malignant T-cell leukemia/lymphoma cells. Oncotarget. 2017 Dec 15;9(5):6259-6269. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9157 mL	19.5787 mL	39.1573 mL	97.8933 mL
	5 mM	0.7831 mL	3.9157 mL	7.8315 mL	19.5787 mL
	10 mM	0.3916 mL	1.9579 mL	3.9157 mL	9.7893 mL
	15 mM	0.2610 mL	1.3052 mL	2.6105 mL	6.5262 mL
	20 mM	0.1958 mL	0.9789 mL	1.9579 mL	4.8947 mL
	25 mM	0.1566 mL	0.7831 mL	1.5663 mL	3.9157 mL
	30 mM	0.1305 mL	0.6526 mL	1.3052 mL	3.2631 mL
	40 mM	0.0979 mL	0.4895 mL	0.9789 mL	2.4473 mL
	50 mM	0.0783 mL	0.3916 mL	0.7831 mL	1.9579 mL
	60 mM	0.0653 mL	0.3263 mL	0.6526 mL	1.6316 mL
	80 mM	0.0489 mL	0.2447 mL	0.4895 mL	1.2237 mL
	100 mM	0.0392 mL	0.1958 mL	0.3916 mL	0.9789 mL

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TJ191

Anti-Blood Cancer Compound Library

Heterocyclic Compound Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

TJ191

Anti-Blood Cancer Compound Library

Heterocyclic Compound Library

Highly Selective Activators Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

TJ191 Related Classifications

HY-120075 Related Classifications