Cy 3 (Non-Sulfonated)
Cy 3 Non-Sulfonated (Cyanine3) is a cyanine (Cy) dye, and a fluorescent label with green channel for protein and nucleic acid. Cy 3 Non-Sulfonated is a fluorescent photoproduct of Cyanine5 via photoconversion upon photoexcitation. Cy 3 Non-Sulfonated can be used to high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives (Ex=470 nm; Em=515 nm and 565 nm nm).
For research use only. We do not sell to patients.
- CAS No.: 146397-20-8
- Formula: C43H50N4O14S2
- Molecular Weight:911.01
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
Labeling of Protein:
1. Add protein (100 μL) to Cy 3 Non-Sulfonated in 96 black micro-well for 50 min at 37 °C. Incubate the cells according to your normal protocol.
2. Scan plate using BioTek and collect data with excitation wavelength of 470 nm, and emission wavelength of 515 nm and 565 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
570
Chemical Information
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CAS No. 146397-20-8
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Molecular Weight 911.01
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Formula C43H50N4O14S2
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SMILES
O=S(C1=CC2=C([N+](CCCCCC(ON3C(CCC3=O)=O)=O)=C(/C=C/C=C4N(CCCCCC(ON5C(CCC5=O)=O)=O)C6=C(C=C(S(=O)(O)=O)C=C6)C/4(C)C)C2(C)C)C=C1)([O-])=O
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Synonyms
Cyanine3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Cho Y, et al. Mechanism of Cyanine5 to Cyanine3 Photoconversion and Its Application for High-Density Single-Particle Tracking in a Living Cell. J Am Chem Soc. 2021 Sep 8;143(35):14125-14135. [Content Brief]
[2]. Li H, et al. Silver enhanced ratiometric nanosensor based on two adjustable Fluorescence Resonance Energy Transfer modes for quantitative protein sensing. Biosens Bioelectron. 2017 Jan 15;87:428-432. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cy 3 (Non-Sulfonated) Related Classifications
HY-D0968 Related Classifications
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