Biotin-itaconate
Based on 1 Customer Validation
Biotin-itaconate is a biotin-labeled itaconic anhydride that targets PD-L1. Biotin-itaconate acts as a biotin probe for in vitro alkylation assays to identify itaconate-modified protein substrates. Biotin-itaconate is applicable to cancer research.
For research use only. We do not sell to patients.
- Purity: 93.23%
- CAS No.: 3086566-93-7
- Formula: C25H42N4O9S
- Molecular Weight:574.69
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Biotin-itaconate (50 μM; 4 h) covalently alkylates the Cys272 residue of wild-type PD-L1 in HEK293T cells, while the C272A mutation abolishes this alkylation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293T cells transfected with hemagglutinin (HA)-tagged wild-type (WT) programmed cell death ligand 1 (PD-L1) or HA-tagged PD-L1 C272A mutant plasmids
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Concentration:50 μM
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Incubation Time:4 h
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Result:Labeled WT HA-PD-L1, as detected by streptavidin blotting of immunoprecipitated protein.
Significantly decreased the level of labeling compared to WT PD-L1 when PD-L1 carried the Cys272 residue mutation.
Chemical Information
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CAS No. 3086566-93-7
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Appearance Oil
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Molecular Weight 574.69
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Formula C25H42N4O9S
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Color Off-white to light brown
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SMILES
OC(C(CC(NCCOCCOCCOCCOCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O)=C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (174.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7401 mL | 8.7003 mL | 17.4007 mL | 43.5017 mL |
| 5 mM | 0.3480 mL | 1.7401 mL | 3.4801 mL | 8.7003 mL | |
| 10 mM | 0.1740 mL | 0.8700 mL | 1.7401 mL | 4.3502 mL | |
| 15 mM | 0.1160 mL | 0.5800 mL | 1.1600 mL | 2.9001 mL | |
| 20 mM | 0.0870 mL | 0.4350 mL | 0.8700 mL | 2.1751 mL | |
| 25 mM | 0.0696 mL | 0.3480 mL | 0.6960 mL | 1.7401 mL | |
| 30 mM | 0.0580 mL | 0.2900 mL | 0.5800 mL | 1.4501 mL | |
| 40 mM | 0.0435 mL | 0.2175 mL | 0.4350 mL | 1.0875 mL | |
| 50 mM | 0.0348 mL | 0.1740 mL | 0.3480 mL | 0.8700 mL | |
| 60 mM | 0.0290 mL | 0.1450 mL | 0.2900 mL | 0.7250 mL | |
| 80 mM | 0.0218 mL | 0.1088 mL | 0.2175 mL | 0.5438 mL | |
| 100 mM | 0.0174 mL | 0.0870 mL | 0.1740 mL | 0.4350 mL |
Biotin-itaconate Related Classifications
HY-185447 Related Classifications
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