Kasugamycin sulfate

Kasugamycin (Ksg) (100 mM) sulfate binds to the Thermus thermophilus 30S ribosomal subunit, forming two binding sites in the mRNA pathway; the primary binding site is located at the top of h44, between h24 and h28, and spans the P/E site region, while the secondary binding site is located adjacent to the E site and interacts with h23, h24 of 16S rRNA and ribosomal protein S7, with the Kasugamycin sulfate-30S complex structure determined at a resolution of 3.35 Å^[2].
Kasugamycin (0-1 mM; 15 min; 37 °C) sulfate inhibits the binding of MVF-mRNA to Escherichia coli 30S ribosomal subunits and 70S ribosomes, with inhibition rates of 40% and 25% at 1 mM, respectively^[2].
Kasugamycin (0-1 mM; 15 min; 37 °C) sulfate inhibits the binding of initiator fMet-tRNA to MVF-mRNA-programmed Escherichia coli 30S ribosomal subunits, with a maximum inhibition rate of 75% at 1 mM, while it has no significant effect on the binding of fMet-tRNA to 70S ribosomes^[2].
Kasugamycin (500 μM; 15 min; 37 °C) sulfate inhibits the binding of leaderless mRNA and initiator fMet-tRNA to Escherichia coli 70S ribosomes by 29% and 13%, respectively, indicating that the 70S initiation complex formed by leaderless mRNA-fMet-tRNA is relatively insensitive to Kasugamycin sulfate^[2].
Kasugamycin (12.5-50 μg/mL) sulfate inhibits 32 strains of Pseudomonas aeruginosa cultured in peptone water at pH 7.0, with 1 strain inhibited at 12.5 μg/mL, 19 strains inhibited at 25 μg/mL, and 12 strains inhibited at 50 μg/mL^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.