PYSNO
PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λem=515-565 nm, λex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases.
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- CAS No.: 2445485-84-5
- Formule: C28H31N5O3S
- Masse moléculaire:517.64
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
In cell-free fluorescence spectroscopy assays, PYSNO (5 μM; 10 min, 60 s, 15 s) rapidly detects NO with a 23-fold fluorescence enhancement, a linear response range of 1 to 30 μM, and a limit of detection of 242 nM[1].
PYSNO (5 μM; 30 min) selectively accumulates in lysosomes of SH-SY5Y human neuroblastoma cells, as confirmed by a Pearson’s colocalization coefficient of 0.87 with Lyso-Tracker Red (HY-D1300) after NO-induced fluorescence activation[1].
PYSNO (5 μM; 30 min) detects exogenous NO in SH-SY5Y human neuroblastoma cells with dose-dependent fluorescence activation, showing a 10.5-fold enhancement at 200 μM NO[1].
PYSNO (5 μM; 30 min) detects endogenously produced NO in RAW264.7 mouse macrophages, showing a 2.8-fold fluorescence enhancement after NO induction, with signal reduction by NOS inhibition or NO scavenging[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice (male, 10 weeks old; myocardial fibrosis induced by subcutaneous isoproterenol injection)[1]
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Dosage:50 μM (200 μL, 0.2% DMSO in PBS)
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Administration:i.v. (tail vein); single dose
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Result:Showed highly enhanced fluorescence in the heart region of myocardial fibrosis model mice relative to normal mice in in vivo imaging.
Confirmed significantly higher fluorescence intensity in myocardial fibrosis model mice relative to normal mice in ex vivo imaging of harvested hearts.
Chemical Information
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CAS No. 2445485-84-5
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Masse moléculaire 517.64
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Formule C28H31N5O3S
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SMILES
NC1=C(C=C(C=C1)C2=CC=C(S2)N3N=C(C=CC3=O)C4=CC=C(C=C4)OCCCCN5CCOCC5)N
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Zhou T, et al. A Smart Fluorescent Probe for NO Detection and Application in Myocardial Fibrosis Imaging. Anal Chem. 2020;92(7):5064-5072. [Content Brief]
[2]. Dong X, et al. Theoretical insights into the linker effects on the turn-on fluorescence behaviors in pyridazinone-containing NO probes. Spectrochim Acta A Mol Biomol Spectrosc. 2024;321:124761. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PYSNO Related Classifications
HY-D3250 Related Classifications
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