1628913-44-9
Chemical Structure
SmBiT Tag
- CAS No.: 1628913-44-9
- Formula:C63H98N14O18
- Molecular Weight:1339.54
InChIKey: QYWNBYQFTPKUPI-WRCYIGFJSA-N
SMILES: O=C(N[C@@H]([C@H](O)C)C(NCC(N[C@@H](CC1=CC=C(C=C1)O)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(C)C)C(N[C@@H](CC2=CC=CC=C2)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCC(O)=O)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CC(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](C(C)C)N
Biological Activity: SmBiT Tag is an 11-amino-acid short peptide with extremely low natural affinity for LgBiT, with a Kd value of approximately 190 μM. It serves as a core component of the NanoLuc Binary Technology (NanoBiT) system. NanoBiT is a bioluminescent reporter system based on protein fragment complementation technology, which is used to monitor dynamic protein-protein interactions in live cells and in vitro[1][2].
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SmBiT Tag | 98.84% | SmBiT Tag is an 11-amino-acid short peptide with extremely low natural affinity for LgBiT, with a Kd value of approximately 190 μM. It serves as a core component of the NanoLuc Binary Technology (NanoBiT) system. NanoBiT is a bioluminescent reporter system based on protein fragment complementation technology, which is used to monitor dynamic protein-protein interactions in live cells and in vitro. | ||||||||||||||||||||
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- [1]. Ji Y, et al. Structural basis of peptide recognition and activation of endothelin receptors. Nat Commun. 2023;14(1):1268. Published 2023 Mar 7. [Content Brief]
- [2]. Zhang CH, et al. Development of a general bioluminescent activity assay for peptide ligases. FEBS J. 2022;289(17):5241-5258. [Content Brief]
Keywords
