SmBiT Tag
Based on 1 Customer Validation
SmBiT Tag is an 11-amino-acid short peptide with extremely low natural affinity for LgBiT, with a Kd value of approximately 190 μM. It serves as a core component of the NanoLuc Binary Technology (NanoBiT) system. NanoBiT is a bioluminescent reporter system based on protein fragment complementation technology, which is used to monitor dynamic protein-protein interactions in live cells and in vitro.
For research use only. We do not sell to patients.
- Purity: 98.84%
- CAS No.: 1628913-44-9
- Formula: C63H98N14O18
- Molecular Weight:1339.54
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
The SmBiT Tag is used in combination with LgBiT, the other core large fragment component of the NanoBiT system. When SmBiT and LgBiT are fused separately to two interacting target proteins, the binding of these two target proteins brings SmBiT and LgBiT close to each other, promoting their complementary assembly, thereby restoring the high activity of NanoLuc luciferase and generating a detectable bioluminescent signal[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1628913-44-9
-
Appearance Solid
-
Molecular Weight 1339.54
-
Formula C63H98N14O18
-
Color White to off-white
-
Sequence
Val-Thr-Gly-Tyr-Arg-Leu-Phe-Glu-Glu-Ile-Leu
-
Sequence Shortening
VTGYRLFEEIL
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
-
Data Sheet (258 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Ji Y, et al. Structural basis of peptide recognition and activation of endothelin receptors. Nat Commun. 2023;14(1):1268. Published 2023 Mar 7. [Content Brief]
[2]. Zhang CH, et al. Development of a general bioluminescent activity assay for peptide ligases. FEBS J. 2022;289(17):5241-5258. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SmBiT Tag Related Classifications
HY-P10633 Related Classifications
Powered by Bioz