76863-28-0
Chemical Structure
Fluorescein-5-thiosemicarbazide
- CAS No.: 76863-28-0
- Formula:C21H15N3O5S
- Molecular Weight:421.43
IUPAC Name: N-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)hydrazinecarbothioamide
InChIKey: OIMMDIXLTJAVTF-UHFFFAOYSA-N
SMILES: NNC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)=S
Biological Activity: Fluorescein-5-thiosemicarbazide is a green fluorescent probe used for carbonyl labeling. The thiosemicarbazide group of FTSC reacts with aldehyde groups at the reducing ends of carbohydrates via reductive amination, and can also undergo chemoselective ligation with ketone groups in systems such as 2-keto Ac4GalNAc to form stable fluorescent derivatives. FTSC is applicable for fluorescent labeling of carbohydrates and enables fluorescence imaging of mucin-type O-linked glycoproteins in the 2-keto Ac4GalNAc metabolic labeling system. Free FTSC exhibits Ex/Em = 495/517 nm in PBS. FTSC is suitable for carbohydrate labeling and cellular glycosylation imaging studies[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Fluorescein-5-thiosemicarbazide | 97.0% | Fluorescein-5-thiosemicarbazide is a green fluorescent probe used for carbonyl labeling. The thiosemicarbazide group of FTSC reacts with aldehyde groups at the reducing ends of carbohydrates via reductive amination, and can also undergo chemoselective ligation with ketone groups in systems such as 2-keto Ac4GalNAc to form stable fluorescent derivatives. FTSC is applicable for fluorescent labeling of carbohydrates and enables fluorescence imaging of mucin-type O-linked glycoproteins in the 2-keto Ac4GalNAc metabolic labeling system. Free FTSC exhibits Ex/Em = 495/517 nm in PBS. FTSC is suitable for carbohydrate labeling and cellular glycosylation imaging studies. | ||||||||||||||||||||
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References
- [1]. Zhang Y, et al. One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells. Carbohydrate research. 2011 Oct 18;346(14):2156-64. [Content Brief]
- [2]. Zhang Y, et al. Fluorescein-5-thiosemicarbazide as a probe for directly imaging of mucin-type O-linked glycoprotein within living cells. Glycoconjugate journal. 2012 Aug;29(5-6):445-52.
