Art. -Nr.: HY-111347

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BB-Cl-Amidine

11 Cited Publications

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Based on 11 publication(s) in Google Scholar

BB-Cl-Amidine is a peptidylarginine deminase (PAD) inhibitor.

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Reinheit: 98.19%
CAS. Nr.: 1802637-39-3
Formel: C₂₆H₂₆ClN₅O
Molecular Weight:459.97

Speicherung:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

BB-Cl-Amidine Chemical Structure

CAS. Nr.: 1802637-39-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

BB-Cl-Amidine is a peptidylarginine deminase (PAD) inhibitor.

IC₅₀ & Target

PAD^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
U2OS	EC₅₀	8.8 μM Compound: 22; BB-Cl amidine	Cytotoxicity against human U2OS cells after 72 hrs by XTT assay Cytotoxicity against human U2OS cells after 72 hrs by XTT assay	[PMID: 28505447]
U2OS	IC₅₀	8.8 μM Compound: 15	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 34520958]

In Vivo

Treatment with BB-Cl-amidine subtly reduces splenomegaly in MRL/lpr mice, while there is a trend towards increased circulating levels of anti-NET antibodies with PAD inhibitor treatment. However, neither PAD inhibitor affected body weight or total IgG levels. Indeed, treatment with both Cl-amidine and BB-Cl-amidine significantly improves endothelium-dependent vasorelaxation. The BB-Cl-amidine group also shows a strong trend towards downregulation of IRGs. Treatment with either Cl-amidine or BB-Cl-amidine significantly improves muzzle alopecia, in many cases preventing it entirely^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

MRL/lpr mice^[1].
Dosage:

1 mg/kg.
Administration:

Subcutaneous injection daily from 8 to 14 weeks of age.
Result:

Significantly improved endothelium-dependent vasorelaxation and showed a strong trend towards downregulation of IRGs.

Chemical Information

CAS. Nr. 1802637-39-3
Appearance Solid
Molecular Weight 459.97
Formel C₂₆H₂₆ClN₅O
Color White to off-white
SMILES

O=C(C1=CC=C(C2=CC=CC=C2)C=C1)N[C@H](C3=NC4=CC=CC=C4N3)CCCNC(CCl)=N
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (11)

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Journal Impact Factor

Nat Cell Biol

Eosinophil extracellular traps drive asthma progression through neuro-immune signals. [Abstract]

2021 Oct;23(10):1060-1072. PMID: 34616019
Nat Commun

Citrullination modulation stabilizes HIF-1α to promote tumour progression. [Abstract]

2024 Sep 3;15(1):7654. PMID: 39227578
Adv Sci (Weinh)

Succinate Dehydrogenase Subunit A (SDHA) Mediated Microglia Extracellular Traps Formation Participating in Cerebral Ischemic Reperfusion Injury. [Abstract]

2025 Aug 20:e11873. PMID: 40832885
Mol Med

Platelet-derived HMGB1 induces NETosis, exacerbating brain damage in the photothrombotic stroke model. [Abstract]

2025 Feb 5;31(1):46. PMID: 39910417
Acta Neuropathol Commun

Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms. [Abstract]

2025 Feb 18;13(1):33. PMID: 39966968
Int Immunopharmacol

HSYA ameliorates venous thromboembolism by depleting the formation of TLR4/NF-κB pathway-dependent neutrophil extracellular traps. [Abstract]

2024 Nov 5;143(Pt 3):113534. PMID: 39504860
Oncol Rep

Inhibition of protein arginine deiminase II suppresses retinoblastoma in orthotopic transplantation in mice. [Abstract]

2023 Jul;50(1):146. PMID: 37326108
J Antibiot (Tokyo)

Antibacterial effects of BB-Cl-Amidine against multidrug-resistant Gram-positive pathogens via membrane disruption. [Abstract]

2025 Oct 9. PMID: 41068498
Cell Physiol Biochem

Peptidylarginine Deiminases Inhibitors Decrease Endothelial Cells Angiogenic Potential by Affecting Akt Signaling and the Expression and Secretion of Angiogenic Factors. [Abstract]

2024 Feb 19;58(1):63-82. PMID: 38374715
bioRxiv

Reciprocal regulation between the protein arginine deiminases and mSWI/SNF chromatin remodelers controls skeletal muscle differentiation and regeneration. [Abstract]

2026 Jan 21:2026.01.20.700682. PMID: 41659480
Res Sq

2024 Jun 04.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (217.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1741 mL

10.8703 mL

21.7405 mL

5 mM

0.4348 mL

2.1741 mL

4.3481 mL

10 mM

0.2174 mL

1.0870 mL

2.1741 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Knight JS, et al. Peptidylarginine deiminase inhibition disrupts NET formation and protects against kidney, skin and vascular disease in lupus-prone MRL/lpr mice. Ann Rheum Dis. 2015 Dec;74(12):2199-206. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1741 mL	10.8703 mL	21.7405 mL	54.3514 mL
	5 mM	0.4348 mL	2.1741 mL	4.3481 mL	10.8703 mL
	10 mM	0.2174 mL	1.0870 mL	2.1741 mL	5.4351 mL
	15 mM	0.1449 mL	0.7247 mL	1.4494 mL	3.6234 mL
	20 mM	0.1087 mL	0.5435 mL	1.0870 mL	2.7176 mL
	25 mM	0.0870 mL	0.4348 mL	0.8696 mL	2.1741 mL
	30 mM	0.0725 mL	0.3623 mL	0.7247 mL	1.8117 mL
	40 mM	0.0544 mL	0.2718 mL	0.5435 mL	1.3588 mL
	50 mM	0.0435 mL	0.2174 mL	0.4348 mL	1.0870 mL
	60 mM	0.0362 mL	0.1812 mL	0.3623 mL	0.9059 mL
	80 mM	0.0272 mL	0.1359 mL	0.2718 mL	0.6794 mL
	100 mM	0.0217 mL	0.1087 mL	0.2174 mL	0.5435 mL

Keywords

BB-Cl-Amidine

11 Cited Publications

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BB-Cl-Amidine

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Publications Citing Use of MedChemExpress (MCE) BB-Cl-Amidine

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BB-Cl-Amidine

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Publications Citing Use of MedChemExpress (MCE) BB-Cl-Amidine

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BB-Cl-Amidine Related Classifications

HY-111347 Related Classifications