Ligustilide
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Ligustilide is is a bioactive phthalide derivative isolated from Angelica sinensis and Chuanxiong. Ligustilide exhibits neuroprotective, anti-cancer, anti-inflammatory, and vasodilator effects.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.06%
- CAS. Nr.: 4431-01-0
- Formel: C12H14O2
- Molecular Weight:190.24
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Speicherung:
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Pretreatment with Ligustilide (1 μM, 5 μM, 15 μM) significantly improves cell viability. The apoptosis rate in glutamate-induced PC12 cells is 13.39%, and decreased in the presence of Ligustilide (1 μM, 5 μM, 15 μM) by 9.06%, 6.48%, 3.82%, separately. Extracellular accumulation of Ca2+ induced by glutamate are significantly reduced by Ligustilide. Pretreatment Ligustilide could decrease the release of cytochrome C from mitochondria, down-regulate Caspase-3 protein expression and up-regulate Bcl-2/Bax ratio, thereby protects PC12 cells from apoptosis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 4431-01-0
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Appearance Liquid (Density: 1.10±0.1 g/cm3)
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Molecular Weight 190.24
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Formel C12H14O2
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Color Colorless to light yellow
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SMILES
O=C1O/C(C2=C1C=CCC2)=C\CCC
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (525.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Eun Sik Choi, et al. Ligustilide attenuates vascular inflammation and activates Nrf2/HO-1 induction and, NO synthesis in HUVECs. Phytomedicine. 2018 Jan 1;38:12-23. [Content Brief]
[2]. Wu Q, et al. Protective effect of ligustilide against glutamate-induced apoptosis in PC12 cells. Yao Xue Xue Bao. 2015 Feb;50(2):162-8. [Content Brief]
[3]. Jing Ma, et al. Ligustilide promotes apoptosis of cancer-associated fibroblasts via the TLR4 pathways. Food Chem Toxicol. 2020 Jan;135:110991. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.2565 mL | 26.2826 mL | 52.5652 mL | 131.4129 mL |
| 5 mM | 1.0513 mL | 5.2565 mL | 10.5130 mL | 26.2826 mL | |
| 10 mM | 0.5257 mL | 2.6283 mL | 5.2565 mL | 13.1413 mL | |
| 15 mM | 0.3504 mL | 1.7522 mL | 3.5043 mL | 8.7609 mL | |
| 20 mM | 0.2628 mL | 1.3141 mL | 2.6283 mL | 6.5706 mL | |
| 25 mM | 0.2103 mL | 1.0513 mL | 2.1026 mL | 5.2565 mL | |
| 30 mM | 0.1752 mL | 0.8761 mL | 1.7522 mL | 4.3804 mL | |
| 40 mM | 0.1314 mL | 0.6571 mL | 1.3141 mL | 3.2853 mL | |
| 50 mM | 0.1051 mL | 0.5257 mL | 1.0513 mL | 2.6283 mL | |
| 60 mM | 0.0876 mL | 0.4380 mL | 0.8761 mL | 2.1902 mL | |
| 80 mM | 0.0657 mL | 0.3285 mL | 0.6571 mL | 1.6427 mL | |
| 100 mM | 0.0526 mL | 0.2628 mL | 0.5257 mL | 1.3141 mL |
Ligustilide Related Classifications
HY-N0401 Related Classifications
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