Art. -Nr.: HY-B1945

DEHP

Name: DEHP
Brand: MedChemExpress
SKU: HY-B1945
Price: 66.00 USD
Availability: InStock
Rating: 5 (13 reviews)

(Synonyms: Bis(2-ethylhexyl) phthalate; Ergoplast FDO; ESBO-D 82)

13 Cited Publications

Kundenbewertung

Based on 13 publication(s) in Google Scholar

DEHP (Bis(2-ethylhexyl) phthalate) is a widely used plasticizer, which has orally active. DEHP can produce a wide spectrum of toxic effects on organisms including neurotoxicity, liver toxicity, immunotoxicity, and reproductive toxicity.

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Reinheit : 99.56%
CAS. Nr.: 117-81-7
Formel: C₂₄H₃₈O₄
Molecular Weight:390.56

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DEHP Chemical Structure

CAS. Nr.: 117-81-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll (3)
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
NCI-H460	IC₅₀	>50 μM Compound: 14	Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay	[PMID: 28606759]

In Vitro

DEHP (0-160 µM, 48 h) dramatically inhibits cell viability in mouse GC-1 spg cells^[1].
DEHP (0-160 µM, 48 h) induces autophagy and apoptosis of mouse GC-1 spg cells via oxidative stress^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Western Blot Analysis^[1]

View Protocol

Cell Line:

Mouse GC-1 spg cells
Concentration:

0-160 µM
Incubation Time:

48 h
Result:

Decreased Bcl-2 level.
Increased the levels of Bax, cleaved Caspase-3, and cleaved Caspase-8.
Increased the levels of LC3-II, Beclin1, and Atg5, as well as the ratio of LC3-II/LC3-I.

In Vivo

DEHP (0-500 mg/kg, p.o., daily, 8 weeks) impairs fertility of female C3H/N mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female C3H/N mice^[1]
Dosage:

0-500 mg/kg
Administration:

p.o., daily, 8 weeks
Result:

Increased the body weight and visceral fat tissue.
Increased the expression of PPARα and PPARγ mRNA at the dose of 500 mg/kg.
Decreased mRNA expression of PPARα transcripts/106 18S molecules at the dose of 500 mg/kg.
Reduced the level of adiponectin mRNA.
Increased the mRNA concentration of FABP4 at the dose of 0.05 mg/kg.

Chemical Information

CAS. Nr. 117-81-7
Appearance Liquid (Density: 0.985 g/cm³)
Molecular Weight 390.56
Formel C₂₄H₃₈O₄
Color Colorless to light yellow
SMILES

O=C(C1=CC=CC=C1C(OCC(CC)CCCC)=O)OCC(CC)CCCC
Synonyms

Bis(2-ethylhexyl) phthalate; Ergoplast FDO; ESBO-D 82
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (13)

All Cell Proliferation/Viability AssayCell Migration/Invasion AssayWBRT-PCRIn Vivo Efficacy Study

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Journal Impact Factor

J Hazard Mater

Di-(2-ethylhexyl) phthalate (DEHP) and its metabolite promote multiple myeloma progression and bortezomib resistance. [Abstract]

2025 Sep 5:495:139147. PMID: 40633344

DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]

DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) enhanced MM cell viability.

DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]

DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) increased the migration and invasion abilities of MM cells.

DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]

DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) elevated levels of cyclin D1, CDK2 and P21 in MM cells.

DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]

DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) increased the transcriptional levels of MYC, SOX2, OCT3/4, NQO1, GCLC, GSTM2, TXNRD1, and HMOX1 in MM cells.

DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]

DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) led to an increase in both tumor weight and volume in the RPMI-8226-derived and U266-derived xenograft mice.
Environ Int

From phthalate exposure to venous thrombotic risk: Decoding the DEHP-ROS mediated OTUD1- p53 stabilization loop in endothelium. [Abstract]

2026 Apr:210:110254. PMID: 41967174
Environ Int

Effects of BBIBP-CorV vaccine on gut microbiota and short-chain fatty acids in mice exposed to bis (2-ethylhexyl) phthalate and dioctyl terephthalate. [Abstract]

2024 Aug:190:108851. PMID: 38941942
Int J Biol Macromol

Di-2-ethylhexylphthalate-induced miR155-5P promotes placental ferroptosis. [Abstract]

2026 May:360:151847. PMID: 41937013
Ecotoxicol Environ Saf

Mechanistic insight into plasticizer di-(2-ethylhexyl) phthalate as an environmental hazard for abdominal aortic aneurysm: Evidence from in vitro and in vivo studies. [Abstract]

2025 Sep 1:302:118689. PMID: 40680446
J Environ Sci

2025 Sep 20.
Chem Biol Interact

Involvement of ductal reaction in Di-(2-ethylhexyl)-phthalate-caused hepatic fibrosis: Molecular mechanisms and potential intervention strategies. [Abstract]

2025 Sep 5:111738. PMID: 40915524
Chem Biol Interact

Mechanisms and targeted prevention of chronic pancreatitis-acinar to ductal metaplasia caused by a low concentration of di-(2-ethylhexyl)-phthalate. [Abstract]

2025 Sep 26:111758. PMID: 41016453
Poult Sci

New insights into DEHP-induced inflammatory injury in chicken spleen: ROS/TLR4/MyD88 pathway and apoptosis/necroptosis-M1 polarization crosstalk. [Abstract]

2025 Jun;104(6):105074. PMID: 40245538
Poult Sci

ROS-mediated M1 polarization-necroptosis crosstalk involved in Di-(2-ethylhexyl) phthalate-induced chicken liver injury. [Abstract]

2025 Jan;104(1):104558. PMID: 39631278
Toxicol Appl Pharmacol

Shared inflammatory targets of phthalate plasticizers in MODS-related syndromes: An integrative network toxicology study with representative DEHP in vitro validation. [Abstract]

2026 Jul:512:117858. PMID: 42107545
Toxicol Appl Pharmacol

Capsaicin alleviates DEHP-induced testicular dysfunction by suppressing oxidative stress in mice. [Abstract]

2026 Jan:506:117655. PMID: 41276025
Sci Total Environ

Di-(2-ethylhexyl) phthalate increases plasma glucose and induces lipid metabolic disorders via FoxO1 in adult mice. [Abstract]

2022 Oct 10:842:156815. PMID: 35750186

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 150 mg/mL (384.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

0.1 M NaOH : 6.67 mg/mL (17.08 mM; ultrasonic and warming and adjust pH to 10 with 0.1 M NaOH and heat to 60°C)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5604 mL

12.8021 mL

25.6043 mL

5 mM

0.5121 mL

2.5604 mL

5.1209 mL

10 mM

0.2560 mL

1.2802 mL

2.5604 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

All Animals and Diseases

Reproductive and Developmental Toxicity Study
Reproductive and developmental toxicity studies detect adverse effects of prenatal or peri/postnatal exposure on maternal condition, pregnancy maintenance, embryo-fetal survival, fetal growth, structural development, and offspring reproductive or developmental endpoints; classic rat protocols generate readouts by comparing treated groups with vehicle, pair-fed, or untreated controls for implantation, resorption, fetal weight, crown-rump length, external morphology, visceral morphology, skeletal ossification, anogenital distance, nipple/areola retention, and postnatal cohort outcomes.
Neurotoxicity Study
This protocol assesses in vitro neurotoxicity by combining neuronal viability, mitochondrial/metabolic activity, neurite outgrowth, and optional neuronal network function readouts. Calcein-AM or resazurin/PrestoBlue readouts estimate viable or metabolically active cells; βIII-tubulin immunofluorescence detects neuronal morphology and neurite networks; TMRE detects mitochondrial membrane potential; and MEA recordings detect functional changes in neuronal network activity.
Hepatotoxicity Study
This protocol evaluates hepatotoxicity using complementary in vivo mouse APAP acute liver injury and in vitro hepatocyte-based cytotoxicity readouts. In vivo APAP injury is assessed by serum ALT/AST, liver histology, hepatic glutathione, protein adducts, necrosis, inflammation, and regeneration-related endpoints. In vitro hepatotoxicity is assessed by loss of viability, leakage of ALT/AST/LDH, oxidative-stress markers, mitochondrial function, nuclear morphology, intracellular calcium, and high-content imaging endpoints.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (622 KB)
- English - EN (622 KB)
- Français - FR (622 KB)
- Deutsch - DE (622 KB)
- Norwegian - NO (622 KB)
- Español - ES (622 KB)
- Swedish - SV (622 KB)
- Italian - IT (622 KB)
- Korean - KR (622 KB)
- Portuguese - PT (622 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Gan Y, et al. Di-2-ethylhexyl phthalate (DEHP) induces apoptosis and autophagy of mouse GC-1 spg cells. Environ Toxicol. 2020 Feb;35(2):292-299. [Content Brief]

[2]. Schmidt JS, et al. Effects of di(2-ethylhexyl) phthalate (DEHP) on female fertility and adipogenesis in C3H/N mice. Environ Health Perspect. 2012 Aug;120(8):1123-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
0.1 M NaOH / DMSO	1 mM	2.5604 mL	12.8021 mL	25.6043 mL	64.0107 mL
	5 mM	0.5121 mL	2.5604 mL	5.1209 mL	12.8021 mL
	10 mM	0.2560 mL	1.2802 mL	2.5604 mL	6.4011 mL
	15 mM	0.1707 mL	0.8535 mL	1.7070 mL	4.2674 mL
DMSO	20 mM	0.1280 mL	0.6401 mL	1.2802 mL	3.2005 mL
	25 mM	0.1024 mL	0.5121 mL	1.0242 mL	2.5604 mL
	30 mM	0.0853 mL	0.4267 mL	0.8535 mL	2.1337 mL
	40 mM	0.0640 mL	0.3201 mL	0.6401 mL	1.6003 mL
	50 mM	0.0512 mL	0.2560 mL	0.5121 mL	1.2802 mL
	60 mM	0.0427 mL	0.2134 mL	0.4267 mL	1.0668 mL
	80 mM	0.0320 mL	0.1600 mL	0.3201 mL	0.8001 mL
	100 mM	0.0256 mL	0.1280 mL	0.2560 mL	0.6401 mL

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DEHP

Publications Citing Use of MedChemExpress (MCE) DEHP

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen