Idesin
Idesin is a compound found in the fruits of Idesia polycarpa. Idesin inhibits LPS (HY-D1056)-induced nitric oxide production with mild cytotoxicity.
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- CAS. Nr.: 20331-85-5
- Formel: C13H18O8
- Molecular Weight:302.28
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| 3T3-L1 | IC50 |
127.2 μM
Compound: 6
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Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry
Antiadipogenic activity in mouse 3T3L1 cells assessed as differentiation of preadipocytes to adipocytes after 8 days by spectrophotometry
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[PMID: 23628332] |
In Vitro
Idesin (Compound 4) (1-100 μM; 25 h) significantly inhibits LPS (HY-D1056)-induced NO production in BV2 microglial cells, with mild cytotoxicity at 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:BV2 mouse microglial cells (LPS-stimulated)
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Concentration:1 μM, 10 μM, 100 μM
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Incubation Time:25 h total (1 h pre-treatment + 24 h co-incubation with LPS)
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Result:Reduced nitrite levels to 7.0 ± 0.1 μM and resulted in 87.2 ± 4.2% cell viability at 1 μM.
Reduced nitrite levels to 5.5 ± 0.3 μM and resulted in 97.6 ± 6.3% cell viability at 10 μM.
Reduced nitrite levels to 4.9 ± 0.5 μM and resulted in 90.7 ± 6.8% cell viability at 100 μM.
Chemical Information
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CAS. Nr. 20331-85-5
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Molecular Weight 302.28
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Formel C13H18O8
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SMILES
OCC(C=CC=C1O)=C1O[C@@H]2O[C@@H]([C@H]([C@@H]([C@H]2O)O)O)CO
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Idesin Related Classifications
HY-N18031 Related Classifications
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