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Art. -Nr.: HY-137316

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Phosphoramide mustard

8 Cited Publications

Kundenbewertung

Based on 8 publication(s) in Google Scholar

Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage.

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CAS. Nr.: 10159-53-2
Formel: C₄H₁₁Cl₂N₂O₂P
Molecular Weight:221.02

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

Phosphoramide mustard Chemical Structure

CAS. Nr.: 10159-53-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage^[1]^[2].

IC₅₀ & Target

DNA Alkylator^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
V79	IC₅₀	77 μM Compound: 27	In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay In vitro cytotoxicity against V-79 chinese hamster lung fibroblasts (3 hr drug exposure time) by clonogenic assay	[PMID: 15239662]

In Vitro

Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication^[1].
Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)^[1].
Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage^[1].
Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

SIGCs
Concentration:

0.5 μM, 1 μM, 3 μM, 6 μM
Incubation Time:

48 hours
Result:

Reduced cell viability at concentrations of 3 μM and higher.

RT-PCR^[1]

Cell Line:

SIGCs
Concentration:

3 μM, 6 μM
Incubation Time:

24 hours, 48 hours
Result:

Increased DDR gene mRNA expression levels.

Western Blot Analysis^[1]

Cell Line:

SIGCs
Concentration:

3 μM, 6 μM
Incubation Time:

24 hours, 48 hours
Result:

Generally increased DDR proteins.

In Vivo

Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats^[2].
Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor^[2]
Dosage:

2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
Administration:

Intraperitoneal injection, once daily, for 5 consecutive days
Result:

Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.

Animal Model:

Rats^[2]
Dosage:

59.4 mg/kg (Pharmacokinetic Analysis)
Administration:

Intravenous injection
Result:

T_1/2 (15.1 min).

Chemical Information

CAS. Nr. 10159-53-2
Molecular Weight 221.02
Formel C₄H₁₁Cl₂N₂O₂P
SMILES

O=P(N(CCCl)CCCl)(N)O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (8)

All Cell Proliferation/Viability AssayBio/Physico-chemical AssayCell Imaging/StainingWB

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Journal Impact Factor

Sci Adv

Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC. [Abstract]

2026 Apr 3;12(14):eaec8684. PMID: 41931605
Stem Cell Res Ther

Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway. [Abstract]

2024 Apr 5;15(1):97. PMID: 38581065
Int J Nanomedicine

Exosomes Derived From Human Mesenchymal Stem Cells Mitigate Follicular Interstitial Cell Ferroptosis via the miR-26a-5p/PTEN/GPX4 Axis in Rats with Chemotherapy-Induced Premature Ovarian Insufficiency. [Abstract]

2025 Aug 22:20:10195-10212. PMID: 40873678
J Funct Foods

2025 Jun.

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Phosphoramide mustard cyclohexanamine (PM) (3–120 μM; 48 h) significantly inhibited Caco-2 cell viability compared with the control-treated group.

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) (PM) promoted the increase of MDA content in Caco-2 cells in a concentration-dependent manner.

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) significantly enhanced the red fluorescence intensity emitted by RhoNox-1 staining, indicating the specific accumulation of Fe²⁺ in Caco-2 cells.

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) treatment of Caco-2 cells caused overexpression of NCOA4 protein in a concentration-dependent manner.

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Phosphoramide mustard cyclohexanamine (PM) (30 μM; 48 h) induced autophagy in Caco-2 cells, as evidenced by increased autophagosomes and autophagolysosomes.
Sci Rep

hUMSCs restore ovarian function in POI mice by regulating GSK3β-mediated mitochondrial dynamic imbalances in theca cells. [Abstract]

2024 Aug 16;14(1):19008. PMID: 39152165
Climacteric

Intravenous administration of mitochondria improves ovarian function by anti-apoptosis in the premature ovarian insufficiency model. [Abstract]

2025 Jan 10:1-12. PMID: 39791362
Tufts University

2025.
SSRN

2024 Mar 21.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258. [Content Brief]

[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Phosphoramide mustard

8 Cited Publications

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Reinheit & Dokumentation
Verweise

1/16
1/16

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Customer Validation
Phosphoramide mustard cyclohexanamine (PM) (3–120 μM; 48 h) significantly inhibited Caco-2 cell viability compared with the control-treated group.
1/16

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Customer Validation
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) (PM) promoted the increase of MDA content in Caco-2 cells in a concentration-dependent manner.
1/16

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Customer Validation
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) significantly enhanced the red fluorescence intensity emitted by RhoNox-1 staining, indicating the specific accumulation of Fe²⁺ in Caco-2 cells.
1/16

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Customer Validation
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) treatment of Caco-2 cells caused overexpression of NCOA4 protein in a concentration-dependent manner.
1/16

Phosphoramide mustard purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.

Customer Validation
Phosphoramide mustard cyclohexanamine (PM) (30 μM; 48 h) induced autophagy in Caco-2 cells, as evidenced by increased autophagosomes and autophagolysosomes.

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Phosphoramide mustard

Publications Citing Use of MedChemExpress (MCE) Phosphoramide mustard

Biologische Aktivität

Chemical Information

Publications (8)

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Phosphoramide mustard Related Classifications

Art. -Nr.: HY-137316